hypermethylation of cpg islands located in the promoter regions of genes is a major event in the development of the majority of cancer types, due to the subsequent aberrant silencing of important tumor suppressor genes. klotho; a novel gene associated primarily with suppressing senescence has been shown to contribute to tumorigenesis as a result of its impaired function. recently the relevance ...

The effect of interferon (IFN)on p11 expression was studied in two human epithelial cell lines (BEAS-2B and HeLa). Treatment with IFNresulted in increased steady-state levels of p11 mRNA and protein expression, with a time-dependent and dose-dependent effect. Transient transfection experiments of a reporter gene construct containing 1498 bp of the 5 -flanking region of the p11 promoter demonstr...

In gastric cancer, altered expression of MUC1, MUC2, MUC5AC, and MUC6 mucin genes has already been described. We show in this report by the means of in situ hybridization, reverse transcriptase-polymerase chain reaction, and transfection assays that MUC5B is also abnormally expressed in gastric carcinomatous tissues and cell lines. We thus undertook to elucidate the molecular mechanisms that re...

Calbindin-D9k (CaBP-9k) binds calcium with high affinity and regulates the distribution of free calcium in the cytoplasm. The expression of CaBP-9k is detected primarily in intestine that is vitamin D target tissue, and accumulates in the enterocytes of the duodenal villi. These enterocytes are the clearest example of vitamin D responsive cells, and the presence of CaBP-9k within them accentuat...

Prolonged (90 minutes) incubation with tumor promoter 12-O-tetnadecanoyl-phorbol-13-acetate (TPA) elicits the redistribution of zinc from the nuclear fraction and mitochondria to the cytosol and microsQmes in rabbit thymocytes. The zinc redistribution might play a role in the TPA-caused desensitization of T lymphocytes towards calcium and in the TPA-induced inhibition of GI--~ S phase transitio...

the comparison of total and ionised serum calcium level in preeclamptic pregnant women and the women with normal pregnancy   z. salari md1*, n. eftekhari md2     1- assistan professor, dept of gynecology, university of medical sciences, kerman, iran 2- associated professor, dept of gynecology, university of medical sciences, kerman, iran     background: preeclampsia remains one of the important...

Stress controls the expression of a cohort of genes. Among these, the glucose-regulated protein (GRP) genes are specifically activated by glucose deprivation, reducing agents, glycosylation block, intracellular calcium or ex vivo incubations of tissues or cells. We demonstrate that these stimuli induce the expression of the cytosolic aspartate aminotransferase gene in adipocytes by a process in...

The cardiac/slow twitch sarcoplasmic reticulum (SR) Ca2+-ATPase gene (SERCA2 ) encodes a calcium transport pump whose expression is regulated in a tissue- and development-specific manner. Previously we have identified two distinct positive regulatory regions (bp -284 to -72 and -1815 to -1105) as important for SERCA2 promoter activity. Here we demonstrate that the SERCA2 distal promoter region ...

Background: The protein of Niemann-pick type C1 (NPC1) gene promotes the egress of cholesterol from late endosomes and lysosomes to other cellular compartments and contributes to a process known as reverse cholesterol transport. This study aimed to examine whether promoter methylation of NPC1 is associated with risk of cardiovascular disease (CVD). Methods: Fifty CVD patients and 50 healthy sub...