In vitro techniques may provide a suitable tool for effective propagation and conservation of plant species representing various ecological niches. The elaboration of such protocols is also prerequisite for selection of heavy-metal-tolerant plant material that could be afterwards used for restoration or remediation of polluted sites. In this study, culture protocol for Gypsophila fastigiata pro...

Metallothioneins belong to a group of intracellular, high molecular andcysteine-rich proteins whose content in an organism increase with increasing concentrationof a heavy metal. The aim of this work was to apply the electrochemical analysis for theanalysis of metallothioneins in earthworms exposed to cadmium ions and brewery sludge.Here we utilized adsorptive transfer technique coupled with di...

Heavy metals are a threat to human health and ecosystem. These days, great deal of attention is being given to green technologies for purification of water contaminated with heavy metal ions. Biosorption is one among such emerging technologies, which utilizes naturally occurring waste materials to sequester heavy metals from wastewater. Cadmium has hazardous impact on living beings; therefore, ...

Among wide spectrum of biomolecules induced by various stress factors low molecular mass protein called metallothionein (MT) is suitable for assessment of the heavy metal environmental pollution. The aim of this work was to determine the metallothionein and total thiols content in larvae of freshwater midges (Chironomus riparius) sampled from laboratory exposure to cadmium(II) ions and from fie...

The Schiff bases (H3L), derived from 2-acetylpyrrole and carbonohydrazide, and its reactions with some transition metal ions (Co(II), Ni(II), Cu(II), Zn(II) and Cd(II)) afforded complexes of formulae [M(H5LA)2(H2O)x]∙2(NO3)∙(CH3CN)z∙(H2O)y-x (M= Co, Ni, Cu or Zn ; 0 ≤ x < y ≤ 3, z = 0 or 1) and {Cd(H4LA)(NO3)}n. These compounds have been characterized by means of elemental analysis, UV–Vis spec...

In our work, we compared the performance of two calix [8] arenes, p-tert-butyl-calix [8] arenetétracétone and p-tert-butyl-calix [8] arene-tetraester for cadmium detection in an aqueous medium. Both sensors were characterized by two electrochemically methods, impedance spectroscopy to evaluate the behavior of the sensitive membrane relative to the cation to be detected and cyclic voltammetry to...

The ligand potassium hydrotris(N- (2,6-xylyl)-2-thioimidazolyl)borate (KTtxylyl) was utilized to construct a carbon paste modified electrode (KTtxylyl/CPE), which studied using electrochemical impedance spectroscopy, cyclic voltammetry and square wave (SWV). Its response investigated, it demonstrated significant improvement in the detection of both lead(II) cadmium(II), allowing determination a...