Background: Corynebacterium glutamicum (C. glutamicum) is a potential host for the secretory production of the heterologous proteins. However, to this date few secretion-type gene expression systems in C. glutamicum have been developed, which limit applications of C. glutamicum in a secretory production of the heterologous proteins.Objectives: In this stu...

genetic typing methods of t. gondii strains have been extensively perfected in recent years. from a technical point of view, many tools usable for genetic studied on single-copy loci have been used: rflp, pcr-rflp, sequencing, rapd-pcr and isoenzyme analysis. we described the cloning and sequence analysis of the gene which encodes the major surface antigen (sag1 or p30) of t. gondii. sag1 is th...

background: lipophosphoglycan 3 (lpg3) is required for the lpg assembly, a well known virulent molecule. in this study, the lpg3 gene of the lizard and mammalian leishmania species were cloned and sequenced. a three-dimensional structure (3d) for the target sequence was also predicted by comparative (homology) modeling. materials and methods: an optimization pcr amplification was performed on g...

let $c$ be a nonempty closed convex subset of a real hilbert space $h$. let ${s_n}$ and ${t_n}$ be sequences of nonexpansive self-mappings of $c$, where one of them is a strongly nonexpansive sequence. k. aoyama and y. kimura introduced the iteration process $x_{n+1}=beta_nx_n+(1-beta_n)s_n(alpha_nu+(1-alpha_n)t_nx_n)$ for finding the common fixed point of ${s_n}$ and ${t_n}$, where $uin c$ is ...

polarization for au/pb(zr ti )o /yba cu o ferroelectric capacitor has been studied and measured by pulse method. a sequence of pulses with amplitudes varying from 0 to 10 volts in 2 volt steps is applied and a conventional polarization curve is obtained by integrating the charge flow from the polarization switching induced by the voltage ramp. a value of 25?c/cm2 is measured for the saturated p...

background: genomic dna was isolated from parabronema skrjabini . rrna region was amplified and sequenced. methods : the rna secondary structure was predicted using mfold software (http://mfold.rit.albany.edu). the secondary structure with bulge, hairpins, helices, interior, external and multi loops was predicted for 5.8srdna of our sequence of p. skrjabini and a sequence of p. skrjabini and tw...