The present study is concerned with the production of glucose oxidase (GOD) from thermophilic Penicillium sp. in a 250 mL shake flask. Fourteen different strains of thermophilic Penicillium sp. were isolated from the soil and were screened for glucose oxidase production. IIBP-13 strain gave maximum extra-cellular glucose oxidase production as compared to other isolates. The effect of submerged ...

The effect on intestinal macromolecular absorption capacity and immunoglobulin G (IgG) transfer of feeding sow colostrum at different intervals and in different quantities to newborn pigs was studied. An amount of 15 ml/kg body weight (BW) colostrum was fed at 3 (treatment 3–15), 6 (treatment 6–15) or 12 (treatment 12–15) h intervals, respectively, starting 0–4 h after birth for 24 h; or 30 ml/...

In a study of microbial utilization of paired hydrocarbon and nonhydrocarbon substrates, an isolate derived from the Mary's River, Benton County, Oregon, was found to have limited growth on glucose alone in a mineral salts medium at 30 C (L. D. Bushnell and H. F. Haas, J. Bacteriol. 41:653, 1944). However, in the presence of 0.5% n-hexadecane (99% purity; Humphrey Chemical Co., North Haven, Con...

In this study we investigated whether the nuclear localization of regucalcin in cloned normal rat kidney tubular epithelial NRK52E cells is regulated after culture with hormonal signaling factors. Stable regucalcin/pCXN2 transfectants with subconfluent monolayers were further cultured for 24 or 48 h in a serum-free medium containing either vehicle, tumor necrosis factor-alpha (TNF-alpha), trans...

A total of 447 cervical or vaginal specimens were inoculated in parallel onto peptone-starch-dextrose (PSD) and Columbia colistin (10 mg/ml)-nalidixic acid (15 mug/ml) (CNA) agar and were incubated for 48 h at 35 degrees C in an atmosphere with 2 to 10% CO2. One hundred (22.4%) of the cultures were positive for Haemophilus vaginalis. Forty-eight of the isolates were recovered from both PSD and ...

Page 24366 Line 4 In a previous study, an apatite (Ap) layer was formed on anodically oxidized titanium (Ti) pins by immersing them in an infusion fluid-based supersaturated calcium phosphate solution at 37 °C for 24 h. In the present study, an Ap layer was also successfully formed using a one-step method at 25 °C for 48 h in an infusion fluid-based supersaturated calcium phosphate solution, wh...

17β-estradiol and testosterone are naturally occurring steroids that co-occur in poultry litter. The effects of litter on sorption of these hormones to soil are not known. Sorption isotherms were developed for C-labeled testosterone and H-labeled estradiol in a Cecil sandy clay loam with and without poultry litter addition. The effect of applying the hormones alone (single-sorbate) or together ...

Thermoelectric (TE) devices have short service lives. These materials undergo thermal degradation at elevated temperatures by processes such as oxidation or sublimation. Our substrates were skutterudite-based TE materials. We covered their surfaces with a liquid high-temperature polymer (HTP)—crosslinked after the deposition, what converted those into solid coatings. Sintering was performed 250...

Carthamus tinctorius Linn, also known as safflower, is a plant with the potential of being used in production antimalarial drugs. The purpose this study was to isolate and identify steroid compounds safflower determine its activity vitro. isolation process conducted through extraction chromatography methods. Then, characterization isolated spectroscopic techniques which include Fourier Transfor...

A normal-phase enantioselective high-performance liquid chromatographic method was developed for the enantiomeric resolution of naproxcinod, the most advanced cyclooxygenase-inhibiting nitric oxide donator of anti-inflammatory drugs designed for treatment of osteoarthritis. The enantiomers of naproxcinod were resolved on a Chiralpak AD-H (250 × 4.6 mm, 5 μm) column using a mobile phase system c...