Dundee, Scotland Grahame Hardie and Dario Alessi have jointly solved a mystery that each had been puzzling over for several years (see ‘The bottom line’ box for a summary of their work). Hardie had been looking for the upstream kinase that regulates the metabolic regulator AMP-activated kinase (AMPK) (see the ‘Background’ box), while only a short walk away Alessi had been searching for the down...

yeasts, especially saccharomyces cerevisiae, are one of the oldest organisms with broad spectrum of applications, owing to their unique genetics and physiology. yeast extract, i.e. the product of yeast cells, is extensively used as nutritional resource in bacterial culture media. the aim of this study was to develop a simple, rapid and cost benefit process to produce the yeast extract. in this ...

Three isoenzymes of brewers’ yeast phosphoglucose isomerase which can be resolved by column chromatography on DEAE-cellulose (NAKAGAWA, Y., AND NOLTMANN, E. A. (1967) J. Biol. Chem. 242, 47824788) have been subjected to physical and chemical characterization. In contrast to the pseudoisoenzymes of rabbit muscle phosphoglucose isomerase (BLACKEWRN, M. N., CHIRGWIN J., M. JAMJZS, G. T., KEMPE, T....

It has been reported that when high-gravity brewers' worts were supplemented with a source of nitrogen and unsaturated lipids and sterol, ethanol concentrations up to 16.4% v/v could be achieved within normal fermentation times. As the resultant harvested yeast can be repitched over a number of generations, there appears to be no reason in industry to limit the gravities of commercial worts to ...

Yeast flocculation is a community-building cell aggregation trait that is an important mechanism of stress resistance and a useful phenotype for brewers; however, it is also a nuisance in many industrial processes, in clinical settings, and in the laboratory. Chemostat-based evolution experiments are impaired by inadvertent selection for aggregation, which we observe in 35% of populations. Thes...

By H. G. PETERING, PH.D., R. A. DELOR, B.S., AND H. C. MURRAY, PH.D. F OLIC acid or vitamin B9 deficiency has been induced in the rat by feeding a purified diet containing succinyl sulfathiazole or sulfaguanidine)’2’3’4’5’6 This deficiency is corrected in either case by feeding free or conjugated pteroyl glutamic acid while the syndrome produced by sulfaguanidine is also corrected by paraaminob...

The utilisation of food production by-products back into within a circular economy is one the driving examples to improve sustainability industry. Brewers spent grain most abundant by-product from brewing industry, with currently it being used as animal feed. In this study, we utilised brewers substrate in solid-state fermentation order produce Type-3 sourdough culture. Sourdough bread becoming...

Today, the Beer Purity Law ( Reinheitsgebot ) is presented as a timeless touchstone of German commercial sentiments, but that was not always case. Until mid-twentieth century, law relatively unknown and unevenly applied across Germany. This began to change thanks market protectionism Bavarian brewers in two conflicts integration between 1950s 1970s. The first sparked by West pitted capital inte...