NADHxbiquinone oxidoreductase (complex I) is the first enzyme in the electron transport chain of mitochondrial respiration and catalyzes electron transfer from NADH to ubiquinone by a large number of redox groups. In bovine heart mitochondria the membrane-bound enzyme is composed of at least 40 subunits, 33 of which are nuclear encoded and 7 of which originate in the mitochondria. The estimated...

From a genomic library of Brassica campestris (brown sarson cv. B54), we have cloned and sequenced about 2 kb of upstream regulatory region from one of the 2S albumin-coding gene family. The sequence has several seed-specific promoter motifs. A sequence alignment of the 5' flanking regions of the available Brassica 2S storage protein genes showed that our sequence is a double crossover recombin...

The genus Brassica includes several important agricultural and horticultural crops. Their current genome structures were shaped by whole-genome triplication followed by extensive diploidization. The availability of several crucifer genome sequences, especially that of Chinese cabbage (Brassica rapa), enables study of the evolution of the mesohexaploid Brassica genomes from their diploid progeni...

Crucifer-feeding flea beetles, Phyllotreta spp., are chronic insect pests in Canadian prairie canola production. Multiple laboratory and field feeding bioassays were conducted to determine the susceptibility of a wide range of crucifer species, cultivars, and accessions to feeding by flea beetles with the goal of discovering sources of resistant germplasm. In 62 bioassays of 218 entries, no con...

A novel subclass of dehydrin genes, homologous to the Raphanus sativus late embryogenesis-abundant (LEA) protein (RsLEA2) and the Arabidopsis thaliana dehydrin, was isolated from Brassica juncea and Brassica napus, here designated BjDHN1 and BnDHN1, respectively. The cDNA of BjDHN1 and BnDHN1 genes share 100% nucleotide identity. The encoded protein is predicted to consist of 183 amino acid res...

Characterization of homoeallelic base-identity in allopolyploids is difficult since homeologous subgenomes are closely related and becomes further challenging if diploid-progenitor data is missing. We present HANDS2, a next-generation sequencing-based tool that enables highly accurate (>90%) genome-wide discovery of homeolog-specific base-identity in allopolyploids even in the absence of a dipl...

Tobacco was transformed with a gene coding for an S-locus-specific glycoprotein of Brassica oleracea. The resulting transgenic plants showed tissue-specific and developmentally regulated expression of the introduced gene. lmmunolocaliration experiments showed that the Brassica gene was expressed in the stylar transmitting tissue of the transgenic plants. The pattern of expression of the introdu...

The Colorado River is contaminated with low levels of perchlorate. Perchlorate has the potential to disrupt thyroid function by inhibiting the uptake of iodide. Brassica are rich sources of thiocyanate and nitrate, also inhibitors of iodide uptake. This study was conducted to estimate potential human exposure to perchlorate, thiocyanate, and nitrate from Brassica sp. irrigated with Colorado Riv...

Detection of phytoplasma classified in phylogenetic group 16SrI, ‘Ca. Phytoplasma asteris’, in the plants of interspecific genotypes of Brassica oleracea x Brassica napus and their back-cross toward B. oleracea (IW7.7xB1) is reported in Poland. The presence of phytoplasmas in leaves of plants showing failure of flower bud formation but not in the asymptomatic hybrids was demonstrated by polymer...