Fatty acid desaturase-2 (FAD2) is a key enzyme in the production of polyunsaturated fatty acids plants. RNAi technology can reduce expression FAD2 genes Brassica napus seeds and acquire transgenic B. plants with high oleic content, but effect seed-specific inhibition on seed metabolites not clear. Here we use widely targeted metabolomics to investigate normal-oleic-acid rapeseed (OA) high-oleic...

Brassica napus is an allotetraploid (AACC) formed from the fusion of two diploid progenitors, Brassica rapa (AA) and Brassica oleracea (CC). Polyploidy and genome-wide rearrangement during the evolution process have resulted in genes that are present as multiple homologs in the B. napus genome. In this study, three B. napus homologous genes encoding endoplasmic reticulum-bound sn-glycerol-3-pho...

BACKGROUND AND AIMS Gene flow from crops to their wild relatives has the potential to alter population growth rates and demography of hybrid populations, especially when a new crop has been genetically modified (GM). This study introduces a comprehensive approach to assess this potential for altered population fitness, and uses a combination of demographic data in two habitat types and mathemat...

Under normal field growth conditions, canola (Brassica napus) seeds produce chloroplasts during early seed development and then catabolize the photosynthetic machinery during seed maturation, producing mature seeds at harvest that are essentially free of chlorophyll (Chl). However, frost exposure early in canola seed development disrupts the normal programming of Chl degradation, resulting in g...

Meiotic crossovers (COs) are essential for proper chromosome segregation and the reshuffling of alleles during meiosis. In WT plants, the number of COs is usually small, which limits the genetic variation that can be captured by plant breeding programs. Part of this limitation is imposed by proteins like FANCM, the inactivation of which results in a 3-fold increase in COs in Arabidopsis thalian...

Introduction Assessment of the mating system of a crop is particularly important for the design of adequate breeding and genetic conservation programs. Genetic inbreeding and heterosis, important factors that affect the production of the crop, are related to the mating system. Brassica napus L. includes economically important crops, such as oilseed rape, rutabaga and leaf rape. B. napus is cons...

The Resistance to Powdery Mildew 8 (RPW8) locus confers broad-spectrum resistance to powdery mildew in Arabidopsis thaliana. There are four Homologous to RPW8s (BrHRs) in Brassica rapa and three in Brassica oleracea (BoHRs). Brassica napus (Bn) is derived from diploidization of a hybrid between B. rapa and B. oleracea, thus should have seven homologs of RPW8 (BnHRs). It is unclear whether these...

Brassica napus cultivar Westar is non-embryogenic under all standard protocols for induction of microspore embryogenesis; however, the rare embryos produced in Westar microspore cultures, induced with added brassinosteroids, were found to develop into heritably stable embryogenic lines after chromosome doubling. One of the Westar-derived doubled haploid (DH) lines, DH-2, produced up to 30% the ...

Seed filling is a dynamic, temporally regulated phase of seed development that determines the composition of storage reserves in mature seeds. Although the metabolic pathways responsible for storage reserve synthesis such as carbohydrates, oils, and proteins are known, little is known about their regulation. Protein phosphorylation is a ubiquitous form of regulation that influences many aspects...

Clubroot disease resistance has been introgressed from Brassica rapa into canola following different approaches including a B. napus × interspecific cross; however, the details of this cross are not available. To extend our knowledge for introgression to multiple pathotypes, we crossed clubroot-resistant turnip with susceptible and backcrossed F1′s canola. The backcross population was subjected...