This study aims to determine the phylogenetic position of rotifer Brachionus sp. sample originated from Tumpaan coastal water, North Sulawesi in tree spp. 27 samples selected 100 available NCBI Genbank. The phylogeny was constructed on basis COI (Cytochrome Oxidase I) gene sequences using MEGA7 software and neighbor-joining method. Results show that divided into 2 clades, group (closely related...