Objective(s): This study describes the preparation, biodistribution of 153Sm-DTPA-SPION after intravenous injection in rats. Materials and Methods: The chelator DTPA dianhydride was conjugated to SPION using a small modification of the well-known cyclic anhydride method. Conjugation was done at a 1: 4 (SPION: ccDTPA) molar ratio. Conjugation reaction was purified with magnetic assorting column...

Our objective was to study the properties of the carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and the methodology of cell labeling using CFDA-SE fluorescent dye. First, we analyzed the kinetics of CFDA-SE fluorescent dye intensity over time. Second, we determined the optimal concentration of CFDA-SE fluorescent dye for cell labeling. Third, we tested the toxicity of CFDA-SE fluores...

Quantum dots emerge as an attractive alternative to small molecule fluorophores as fluorescent tags for in vivo cell labeling and imaging. This communication presents a method for specific labeling of live cells using quantum dots. The labeling is mediated by HaloTag protein expressed at the cell surface which forms a stable covalent adduct with its ligand (HaloTag ligand). The labeling can be ...

for a given graph $g=(v,e)$, let $mathscr l(g)={l(v) : vin v}$ be a prescribed list assignment. $g$ is $mathscr l$-$l(2,1)$-colorable if there exists a vertex labeling $f$ of $g$ such that $f(v)in l(v)$ for all $v in v$; $|f(u)-f(v)|geq 2$ if $d_g(u,v) = 1$; and $|f(u)-f(v)|geq 1$ if $d_g(u,v)=2$. if $g$ is $mathscr l$-$l(2,1)$-colorable for every list assignment $mathscr l$ with $|l(v)|geq k$ ...

Abstract Cell therapy is an emerging field that uses cells as living drugs to treat a broad array of acute and chronic diseases. Most cell therapies in clinical trials are made using standard bench methods, whose open processing require manufacturing expensive GMP cleanrooms. As progress, new methods needed enable scalable while maintaining process integrity, reducing environmental exposure, li...

The ability to form anastomoses with the host circulation is essential for vascular networks incorporated within cell-seeded bioengineered tissues. Here, we tested whether and how rapidly human endothelial colony forming cell (ECFC)/mesenchymal progenitor cell (MPC)-derived bioengineered vessels, originally perfused in one mouse, could become reperfused in a secondary mouse. Using in vivo label...

In this paper we generalize the remainder cordial labeling, called $k$-remainder cordial labeling and investigate the $4$-remainder cordial labeling behavior of certain graphs.

HE DIFFERENTIAL AGGLUTINATION METHOD’ of determining I the life-span of the erythrocyte has been applied to the study of sickle cell anemia,2’3 sickle-cell/Hb-C disease46 and pure ( homozygous ) hemoglobin-C disease.7’8 In this way the survival time of sickle cells was found to be shortened whether transfused to normal recipients, to recipients with sickle cell trait. or to recipients with sick...