Dear Editor, Carbapenems are considered as the last resort of antibiotic for the treatment of infection but many Gram-negative organisms have developed resistance to this antibiotic through loss or alteration of outer membrane porin protein OprD, over-expression of efflux pump, hyperproduction of an AmpC-type-β-lactamase and/or carbapenemase.1 Carbapenemases are β-lactamases with catalytic effi...

ICUs are crucial medical facilities for treating life-threatening diseases, and bacterial contamination of ICU equipment devices is a significant cause nosocomial infections. In this study, total 100 clinical specimens (urine, sputum pus) were collected from patients admitted in the ICU. Results showed eighty two positive growth culture. 30(36.5%) female and‎‏‎ 52( 63.5%) male ‎ to intensive ca...

328 May 2015, Vol. 105, No. 5 VIM-2 carbapenemase-producing Pseudomonas aeruginosa in a patient from Port Elizabeth, South Africa To the Editor: We report on the emergence of a multidrug-resistant Pseudomonas aeruginosa isolate in a public hospital in Port Elizabeth, Eastern Cape Province, South Africa (SA), where the lack of antibiotic stewardship may have been a contributing factor. A 76-year...

A plasmid-encoded class II transposon element was identified in a carbapenem-resistant Pseudomonas putida isolate. Tn1332, closely related to Tn1331, harbored the metallo-beta-lactamase gene bla(VIM-2) in addition to four other antibiotic resistance genes, aacA4, aadA1, bla(OXA-9), and bla(TEM-1), and two novel insertion sequences, ISPpu17 and ISPpu18.

Pseudomonas aeruginosa (P. aeruginosa) places among major opportunistic nosocomial pathogen which has developed extensive drug resistance. Due to uncontrolled consumption of antibiotics, multidrug-resistant (MDR) P. species are increasingly isolated from various settings hospitals globally. This research aimed determine the genes reproducing blaOXA-48, blaIMP-1, blaVIM-1 and blaNDM-1 metallo be...