Although biuret based protein assays are theoretically applicable to peptide measurement, there is a high level of inter-peptide variation, determined largely by peptide hydrophobicity. This variation in peptide reactivity can be significantly reduced by heat-denaturation of peptides at 95 °C for 5 minutes in the presence of 0.1 M NaOH containing 1% (w/v) SDS, prior to incubation for 30 min at ...

Introduction: Fish innards contain 14.01% protein, 20% lipid, 4.75% ash, and 60.62% water. are formulated into granules for practicality in their application as animal feed. 
 Aim: This research on the proximate analysis of feed composed raw material from fish wastes used a descriptive quantitative method. Results: The result indicated that water content measured using thermogravimetric me...

Bromelin merupakan unsur pokok dari nanas yang penting dan berguna dalam bidang farmasi makanan, untuk mendapatkan enzim bromelin buah diperlukan proses isolasi bromelin. Tujuan penelitan ini adalah mengisolasi mengkarakterisasi serbuk batang (Ananas Comosus (L.) Merr). Isolasi telah dilakukan dengan menggunakan pelarut dapar fosfat pH 7.0 disentrifugasi pada 3.500 rpm selama 15 menit. Serbuk d...

Enzymatic activity was determined in an optical test, based on the measurement of reduced DPN at 340 rnp in the Beckman spectrophotometer. The reaction was carried out in phosphate or cysteine buffer at pH 7. Synthetic dihydroxyacetone phosphate (5) with reduced DPN or racemic cr-glycerophosphate with DPN was used as substrate to measure the forward and backward reactions, respectively. The con...

Two experiments were conducted to determine the influence of supplemental nonprotein N (NPN) provided daily (D) or every other day (2D) on ruminant performance and N efficiency. Treatments included an unsupplemented control (CON) and a urea (28.7% CP) or biuret (28.6% CP) supplement provided D or 2D at 0700. In Exp. 1, five wethers (39 +/- 1 kg BW) were used in an incomplete 5 x 4 Latin square ...