Human campylobacteriosis, an infection caused by the bacterium Campylobacter, is a major issue in the United States food system, especially for poultry products. According to the Center for Disease Control, campylobacterosis is estimated to affect over 2.4 million people annually. Campylobacter jejuni and Campylobacter coli are 2 species responsible for the majority of campylobacterosis infecti...

The ability of Branhamella catarrhalis to cause nosocomial infections is a matter of some controversy. The API ZYM research kit for detecting 89 enzymes was used on 49 isolates of B catarrhalis to select enzymes of potential use in differentiating clinical isolates. Twenty nine enzymes were produced by all isolates (13 strongly positive) and many of these were esterases; 16 enzymes were not det...

Amplified fragment length polymorphisms (AFLPs) were used to characterize the genotypic diversity of a total of 114 Gallibacterium anatis isolates originating from a reference collection representing 15 biovars from four countries and isolates obtained from tracheal and cloacal swab samples of chickens from an organic, egg-producing flock and a layer parent flock. A subset of strains was also c...

The main object of the study is to demonstrate that Bruce-ladder multiplex PCR, which capable identify all Brucella species including vaccinal strains, a safe and practical method alternative bacteriological culture methods. Brucellosis zoonosis great socio-economic importance endemic in Turkey. In three-year period from 2009 2011, total 281 spp. isolates cattle, sheep goats were identified cha...

Campylobacter pylori (C. pyloridis) is a fastidious organism found in the gastric mucosa associated with histological gastritis and peptic ulceration. A rapid identification scheme that detects the presence of preformed enzymes (Rosco Diagnostica, Taastrup, Denmark) was applied to clinical isolates of C. pylori. The isolates tested were a very homogeneous group. They all produced oxidase, catal...

Forty-five enteropathogenic (enteropathogenic Escherichia coli-like) strains isolated in commercial rabbit farms were subdivided into four biotypes with the help of six carbohydrate fermentation tests, ornithine decarboxylase tests, and motility tests. E. coli strains which caused neonatal diarrhea in baby rabbits belonged to a single biotype, biotype 1, and displayed the same serotype antigens...

Three commercially available systems, the 4-h Minitek Enterobacteriaceae III, the Haemophilus Trio-Tube, and the Micro-ID, were evaluated for their capacities to identify and biotype 308 respiratory isolates of Haemophilus spp. When compared with aminolevulinic acid test results, the definitive identification method used in this study, these systems demonstrated no significant differences in th...

Two hundred and twelve Micrococcaceae isolates were obtained from 82 men with nongonococcal urethritis, 24 women with vaginitis, and 54 girls with vulvovaginitis. Identification and biotyping of these strains were carried out by using the simplified scheme of Kloos and Schleifer (W. E. Kloos and K. H. Schleifer, J. Clin. Microbiol. 1:82-88, 1975) and the commercially available API Staph test (D...

Multi-drug resistant (MDR) strains of Acinetobacter baumannii are responsible for an increasing number of opportunistic infections in hospitals. This study determined the prevalence of MDR A. baumannii isolates from intensive care units in a large tertiary-care hospital in Ismailia, Egypt, and the occurrence of different beta-lactamases in these isolates. Biotyping and antimicrobial susceptibil...

The genus Listeria is composed of six species of which Listeria monocytogenes is considered the single pathogenic species that causes listeriosis in humans. Of the 13 serovars of L. monocytogenes, 1/2a, 1/2b and 4b are responsible for the majority of clinical cases. The aim of this work was to detect L. monocytogenes in the cerebrospinal fluid sample of premature newborns and to characterize th...