نتایج جستجو برای: biotinylated hyaluronan probe bha
تعداد نتایج: 101682 فیلتر نتایج به سال:
Hyaluronan-binding sites were demonstrated on the cell surface of three malignant mesothelioma cell lines derived from human tumors using either [3H]hyaluronan or fluorescein-tagged hyaluronan. No hyaluronan-binding activity was observed on normal human mesothelial cells. The absence of hyaluronan receptors on normal human mesothelial cells was not due to a down-regulation by endogenously synth...
Nasopharyngeal carcinoma, particulary tumors endemic to the Far East, commonly harbors Epstein-Barr virus. The detection of nuclear antigen associated with EBV and viral DNA in NPC cells have revealed that EBV can infect epithelial cells and is associated with transformation. Human papilloma virus is an epitheliotrophic oncogenic virus that has been detected in a variety of head and neck tu...
This report describes the use of fluorescence in situ hybridization for chromosome classification and detection of chromosome aberrations. Biotin-labeled DNA was hybridized to target chromosomes and subsequently rendered fluorescent by successive treatments with fluorescein-labeled avidin and biotinylated anti-avidin antibody. Human chromosomes in human-hamster hybrid cell lines were intensely ...
We have developed a general procedure for the rapid and efficient enrichment of specific DNA, RNA or cDNA sequences. Biotinylated DNA or RNA is used as a hybridization probe in solution, avidin is then added to label both the probe and hybrid molecules, and the hybridization mixture chromatographed over cupric-iminodiacetic acid agarose beads. Avidin-probe and avidin-hybrid molecules are select...
Hyaluronan is a biologically active polymer, which can be formulated into nanoparticles. In our study, we aimed to probe atherosclerosis-associated inflammation by using hyaluronan nanoparticles and to determine whether they can ameliorate atherosclerosis. Hyaluronan nanoparticles (HA-NPs) were prepared by reacting amine-functionalized oligomeric hyaluronan (HA) with cholanic ester and labeled ...
To investigate the roles of hyaluronan produced by cancer cells in cancer metastasis, the metastatic potential of the highly metastatic mouse mammary carcinoma FM3A HA1 cell line was compared with those of hyaluronan-deficient mutant cells. Five different mutant clones showed markedly reduced hyaluronan production and lacked the ability to form hyaluronan-rich pericellular coats. These mutant c...
Effects of feeding mice and rats with 2(3)-ferf-butyl-4-hydroxyanisole (BHA) and 3,5-di-ferf-butyl-4-hydroxytoluene (BHT), the two most commonly used food-additive phenolic antioxidants with known anticarcinogenic properties but with only minor differences in their chemical structures, have been compared to search for common effects between the two agents in two different rodent species and the...
Administrationof the antioxidant 2(3)-tert-butyl-4-hy droxyanisole(BHA)in the diet causeda markedincrease in the specific activity of epoxide hydratase (EC 4.2.1.63) in hepatic microsomesof CD-i mice. The increases in epoxide hydratase activities produced by BHA were far greater (11-fold)than were those producedby the admin istrationof well-knownenzymeinducerssuchas 3-meth ylcholanthrene,phenob...
In situ hybridization coupled with electron microscopy has been used to locate mRNAs for the small and large subunits of ribulose 1,5-bisphosphate carboxlase in young leaf tissue of tobacco (Nicotiana tabacum L.) plants. The endogeneous mRNAs were hybridized with either a biotinylated DNA probe for the small subunit or large subunit and subsequently visualized using avidin-ferritin conjugates a...
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