the present study investigated the isolation and identification of biosurfactant producing bacteria from iranian oil wells. the biosurfactant production of bacteria isolates was evaluated and confirmed using hemolysis and emulsification tests. the biodegradation of crude oil was studied using gc and hplc analysis. a total of 45 strains have been isolated. these strains showed less than a 40 mn ...

Multifarious applications of Bacillus licheniformis VS16-derived biosurfactant were explored. Labeo rohita fingerlings were injected intraperitoneally with 0.1 mL of phosphate-buffered saline (PBS) containing purified biosurfactant at 0 (control), 55 (S55), 110 (S110), 220 (S220), or 330 (S330) μg mL-1 concentrations. Various immunological parameters and the expression of immune-related genes w...

The current study evaluated hydrocarbon-degrading and biosurfactant-producing potentials of bacteria isolated from hydrocarbon contaminated soil in Nigeria, the largest crude oil reservoir Africa. Pure bacterial isolates were grown on nutrient Bushnell Haas agar. Bacterial that grew both media molecularly identified via 16S rDNA sequence. Biosurfactant production detection was carried out sprea...

Lactic acid bacteria are generally believed to have positive roles in maintaining good health and immune system in humans. A number of Lactobacilli spp. are known to produce important metabolites, among which biosurfactants in particular have shown antimicrobial activity against several pathogens in the intestinal tract and female urogenital tract partly through interfering with biofilm formati...

In situ biosurfactant (rhamnolipid) production by Pseudomonas putida CB-100 was achieved during a bioaugmented and biostimulated treatment to remove hydrocarbons from aged contaminated soil from oil well drilling operations. Rhamnolipid production and contaminant removal were determined for several treatments of irradiated and non-irradiated soils: nutrient addition (nitrogen and phosphorus), P...

In this paper, an air isolate (NITT6L) has been screened based on hemolytic activity, emulsification activity, drop collapsing test, and oil displacement test, as well as lipase activity. It was found that strain NITT6L was able to reduce the surface tension of the medium from 61.5 to 39.83 mN/m and could form stable emulsions with tested vegetable oils. Morphological, biochemical, 16S rRNA seq...

Biosurfactants are widely known as surface active agents of biological origin. Glycolipid classes of biosurfactants has high important in the biotechnological arena. Pseudomonas aeruginosa, Bacillus subtilis, and Candida sp., are important classes of microorganism and highly investigated for the production of glycolipid biosurfactants. Commercially, microbial biosurfactants are more advantageou...

During the last years, several applications of biosurfactants with medical purposes have been reported. Biosurfactants are considered relevant molecules for applications in combating many diseases. However, their use is currently extremely limited due to their high cost in relation to that of chemical surfactants. Use of inexpensive substrates can drastically decrease its production cost. Here,...

The present study is an attempt to optimize simultaneous production of keratinolytic protease, amylase and biosurfactant from feather meal, potato peel and rape seed cake in a single media by response surface methodology to evaluate their biochemical properties for detergent additive. The optimization was carried out using 20 run, 3 factor and 5-level of central composite design on design exper...

Biosurfactant production by some bacterial isolates using molasses, milk whey and cassava flour wastewater (manipueira) as substrates was evaluated and compared with the production in conventional medium. Isolates growing in manipueira medium decreased the surface tension around 42%, the highest reduction among all the substrates tested. From the eleven isolates tested, eight were able to decre...