The function and activity of a protein are often modulated by other proteins with which it interacts. This review is intended as a practical guide to the analysis of such protein-protein interactions. We discuss biochemical methods such as protein affinity chromatography, affinity blotting, coimmunoprecipitation, and cross-linking; molecular biological methods such as protein probing, the two-h...

A structural and biochemical study is presented concerning the agglutination of gametic flagella, the initial step in the mating reaction of Chlamydomonas reinhardtii. An alteration in the distribution of the intramembranous particles revealed by freeze-fracturing of flagellar membranes is shown to accompany gametic differentiation in both mating types. The isolation and electrophoretic analysi...

Lohmann and Meyerhof first differentiated, in rabbit muscle extracts, the action of enolase from that of phosphoglyceromutase and pyruvic acid kinase (1). Since that time enolase has been crystallized from yeast by Warburg and Christian (2) and extensively investigated, but the enzyme bearing the same function in muscle tissue has received comparatively little study. A preparation of crystallin...

Microtubule-associated proteins can influence the organization, stability and dynamics of microtubules. We characterize a novel protein that associates with microtubules as assessed by immunofluorescence, immunoelectron microscopy, and co-sedimentation. The protein is expressed heavily in embryonic neurons and, to a lesser extent, in epithelial and mesodermal cells. The cDNA sequence predicts a...

in this study, the concentrations of heavy metals (ca, zn, cu, fe, mn, ni) in thesediment of shavoor river in khuzestan province in iran has been investigated. after the library studies and field studies, six samples of water and sediment were taken from the river in order to evaluate heavy metal pollution in sediments. to determine the geochemical phases of metals in sediment samples the 5-ste...

Low insecticide exposure has been shown to cause profound effects on non-target organisms, including crabs. Therefore, the changes in hematological parameters, serum biochemistry and antioxidant enzymes Gercacinid crab, Cardiosoma armatum were assessed during 28-day four concentrations of organophosphate chlorpyrifos (0.003, 0.006, 0.03 0.06 mg/l). The results showed a significant (P= 0.0) decr...

In this study, the procedure of obtaining new biologically active mannoprotein preparation based on yeast biomass from residues wine industry, namely production Merlot dry red is presented. The process for includes lysis cell wall with sodium phosphate buffer, extraction anthocyanins by treatment 50% alcohol solution, hydrolysis 1N NaOH, sedimentation mannoproteins ethyl alcohol, centrifugation...

This is the case report of a 39-year-old lady who presented with clinical and laboratory features secondary hypothyroidism two years after achieving biochemical resolution Graves' disease cessation anti-thyroid medications. The thyroid function tests at presentation revealed: Serum T3 1.33ng/ml (normal range 0.8-1.7ng/ml), T4 2.92 4.5-12.0µg/dl), TSH 0.2mIU/l 0.5-5.0mIU/l). Thyroid-related auto...

The dynamics of a flexible filament sedimenting in a viscous fluid are explored analytically and numerically. Compared with the well-studied case of sedimenting rigid rods, the introduction of filament compliance is shown to cause a significant alteration in the long-time sedimentation orientation and filament geometry. A model is developed by balancing viscous, elastic and gravitational forces...

Certain factors are common to the erythrocyte sedimentation rate and the plasma viscosity. Thus fibrinogen increases the erythrocyte sedimentation rate (Gram, 1921 ; Greisheimer, Johnson, and Ryan, 1929) and Hardwicke, Ricketts, and Squire (1950) found that a direct logarithmic relation exists between the erythrocyte sedimentation rate and the plasma fibrinogen concentration. Also fibrinogen ex...