Recent advancements in 3D bioprinting have led to the fabrication of more complex, more precise, and larger printed tissue constructs. As the field continues to advance, it is critical to develop quantitative benchmarks to compare different bio-inks for key cell-biomaterial interactions, including (1) cell sedimentation within the ink cartridge, (2) cell viability during extrusion, and (3) cell...

The identification of recent HIV infection is important for epidemiological studies and to monitor the epidemic. The objective of this study was to evaluate two rapid tests that are easily available to the Brazilian scientific community for using as markers of recent HIV infection. The Rapid Test - HIV-1/2 Bio-Manguinhos (Bio-Manguinhos/Fiocruz, Brazil) and the Rapid Check HIV 1&2 (NDI-UFES, Ce...

Investigations on susceptibility of insecticides in cotton mealybug (Phenacoccus solenopsis Tinsley) was carried out at Main Cotton Research Station, Navsari Agricultural University, Surat during October 2020 to January 2021 through the IRAC leaf dip bio-assay technique. Mealybug population from farmer’s field Bharuch district were collected and reared NAU, under cage cover. Leaf bio-assays for...

The principle of three-dimensional (3D) bio-printing involves integration biomaterials, live cells and controlled motor systems for creating complex biomimetic constructs. Bio-ink is one the most important components in process 3D needs to be sufficiently viscous dispensed as a free-standing filament but biocompatible maintain cell viability function. Alginate has been used widely due its bioco...

A general method is described to analyze pathogen DNAs in homogenous solution by a novel Bio-MassCode probe, using a small organic (namely disulfide) "reporter mass" that self-assembled in oligonucleotide-modified gold nanoparticles. With conventional MALDI TOF MS, the assay simultaneously detected DNAs for HIV, HBV, HCV, and TP with extraordinarily high sensitivity in the low attomolar (10(-18...

Among the current activities of the IAEA laboratory is a project for the measurement and analysis of small amounts of radioactivity in living persons. This is done with what is known as a "whole body counter" installed in the basement of the Agency's headquarters building in Vienna. This facility is also available for rendering services to Member States at their request as part of the bio-assay...

Biotin is one of the most useful tags in (bio)analytical science due to its specific interaction with avidin, but is not easy to convert because of its low solubility in most solvents. Friedel-Crafts acylation of biotin acid chloride in triflic acid was examined, and the synthesized derivatives had stronger affinity to avidin than biotin in a binding assay using 2-(4'-hydroxyphenylazo)benzoic a...

was quantified by integration using EZLogicTM integration software (Bio-Rad). The clearance of host cell proteins (HCP) and double-stranded DNAs (dsDNA) was determined by a CHO-CM HCP ELISA kit (Cygnus Technologies) and Quant-iT dsDNA high-sensitivity assay kit (Life Technologies Corporation), respectively. mAb1 concentration was determined by UV absorption at 280 nm using a coefficient of 1.4 ...