Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) possesses both growth-inhibitory and -potentiating effects on cells that are independent of IGF action and are mediated through specific IGFBP-3 binding proteins/receptors located at the cell membrane, cytosol, or nuclear compartments and in the extracellular matrix. We have here characterized transferrin (Tf) as one of these IGFBP-3 ...

background: bacterial superantigen staphylococcal enterotoxins (ses), has stimulated polyclonal t cells irrespective of their antigen specificity, resulted a massive release of cytokines, and suggested that they could be assigned as a candidate of new antitumor agents. recent attempts have done to specifically target superantigens towards tumors, subsequently monoclonal antibodies and tumor-rel...

Seek a cell anchorage galectin bridge: Transforming the wildtype Galectin (Gal)-1 and -3 to tandem-repeat type variant via rational protein engineering enhanced cis-binding activity with O-Mannosylated core M1 glycopeptides of α-dystroglycan (DG). Furthermore, Gal-1 variants (but not Gal-3 variants) demonstrated trans-bridging prepared α-DG core-M1 glycoconjugates laminins in situ carbohydrate-...

objective: druggability of a target protein depends on the interacting micro-environment between the target protein and drugs. therefore, a precise knowledge of the interacting micro-environment between the target protein and drugs is requisite for drug discovery process. to understand such micro-environment, we performed in silico interaction analysis between a human target protein, dipeptidyl...

human serum albumin (hsa) is an abundant, multifunctional and nonglycosylated negatively charged plasma protein. hsa ascribed ligand-binding and transport properties, antioxidant functions and enzymatic activities. in the present study, the interaction and side effects of a new designed anti-cancer compound (1,10-phenanthroline butyl dithiocarbamato palladium(ii) nitrate) on hsa have been inves...

background: recently, botulinum neurotoxin (bont)-derived recombinant proteins have been suggested as potential botulism vaccines. here, with concentrating on bont type e (bont/e), we studied two of these binding domain-based recombinant proteins: a multivalent chimer protein, which is composed of bont serotypes a, b and e binding subdomains, and a monovalent recombinant protein, which contains...

background: recent research on several dna fragments covering open reading frames (orf) 1-37 shows a new genetic marker in orf 6 which is specific for differentiating wild-type varicella-zoster virus (vzv) strains from oka varicella vaccine strain. on the other hand, herpes simplex virus (hsv) genome analysis by restriction enzymes is used to differentiate types one and two of the virus and eve...

because of participation in many aspects of human life, and due to oxidation-sensitive characteristics of dopamine (da) and arachidonoyl dopamine (aa-da), the necessity of biocompatible carrier to keep them against oxidation is of importance. in this work, we explored the putative binding sites of da and aa-da to -lactoglobulin (blg) as potent carrier. docking results identified the binding si...