Bovine retinal vessels have sites that specifically bind 3H-p-aminoclonidine (3H-PAC) with an apparent dissociation constant of 0.12 nM and a capacity of binding of 0.15 pmol/g. In addition, these vessels have 3H-prazosin binding sites bearing a dissociation constant of 5 nM and a binding capacity of 5 pmol/g. To understand the implications for retina-optic nerve vascular physiology and pathoph...

Colchicine forms a complex in vivo with a protein present in fertilized or unfertilized sea urchin eggs; similar binding was obtained in vitro with the soluble fraction from egg homogenates. Kinetic parameters and binding equilibrium constant were essentially the same in vivo and in vitro. The binding site protein was shown to have a sedimentation constant of 6S by zone centrifugation. The prot...

Recently we found that Ca2+ within chromaffin vesicles is largely bound [Bulenda, D., & Gratzl, M. (1985) Biochemistry 24, 7760-7765]. In order to explore the nature of these bonds, we analyzed the binding of Ca2+ to the vesicle matrix proteins as well as to ATP, the main nucleotide present in these vesicles. The dissociation constant at pH 7 is 50 microM (number of binding sites, n = 180 nmol/...

background some herbal anticancer agents have direct interactions with dna. objectives the present study aimed to investigate the interaction of ctdna with rosemary flowers. materials and methods we used uv-vis, fluorescence and circular dichroism (cd) spectroscopic techniques. results the absorption of dna at 260 nm increased on addition of rosemary. herb extract quench the fluorescence of etb...

Colchicine forms a complex in vivo with a protein present in fertilized or unfertilized sea urchin eggs; similar binding was obtained in vitro with the soluble fraction from egg homogenates. Kinetic parameters and binding equilibrium constant were essentially the same in vivo and in vitro. The binding site protein was shown to have a sedimentation constant of 6S by zone centrifugation. The prot...

Clinically healthy adult black Bengal female goats were used to investigate the pharmacokinetics of closantel following intravenous administration (10 mg/kg) both in healthy and in liver damaged goats. Induction of liver damage was done by subcutaneous injection of carbontetrachloride at 0.75 mL/kg on three occasion at 48 h interval. After administration of drug, blood samples were collected in...

The binding of thyroxine by bovine and human serum albumin has been studied by measurements of the quenching of the ultraviolet fluorescence of the protein. The results obtained by this method have been compared with those of equilibrium dialysis measurements. Both approaches agree in assigning to serum albumin a single binding site of high affinity and three or more weak sites. The binding con...