In a previous paper (XLVI of this series), the isolation of cyprinol and other three new bile sterols from the alkaline hydrolyzate of carp bile was reported. Also it was suggested that the chemical constitution of one of these new bile sterols would correspond to 3ƒ¿, 7ƒ¿, 12ƒ¿-trihydroxy-26, 27-epoxycholestane and the natural form of this new bile sterol might be 3ƒ¿,7ƒ¿,12ƒ¿,26,27-pentahydro...

BACKGROUND The colon plays a key role in regulating the homeostasis of bile acids. AIM The present study aims to evaluate the influence of colon cancer towards the homeostasis of bile acids. METHODS The free and conjugated bile acids were determined using ultraperformance LC (UPLC) coupled with ABI 4000 QTRAP triple quadrupole instruments. RESULTS The results showed that the free bile aci...

Gallbladder bile of the bullfrog, R a m catesbezana was found to contain a number of minor bile acids and bile alcohols in unconjugated forms. The following bile acids and bile alcohols were identified by combined gas-liquid chromatography-mass spectrometry: cholic acid, allocholic acid, 3a,7a, 12a-trihydroxy-5a-cholestan-26-oic acid, 3a,7a, 12atrihydroxy-24-methyl-5~-cholestan-26-oic acid, 3a,...

Mice lacking the nuclear bile acid receptor FXR/BAR developed normally and were outwardly identical to wild-type littermates. FXR/BAR null mice were distinguished from wild-type mice by elevated serum bile acid, cholesterol, and triglycerides, increased hepatic cholesterol and triglycerides, and a proatherogenic serum lipoprotein profile. FXR/BAR null mice also had reduced bile acid pools and r...

The total content and profile of bile salts and phospholipids are reported for several mammalian biles. Rabbit and guinea-pig biles are characterized by high proportions of conjugated dihydroxy bile salts with respect to trihydroxy bile salts, but contain relatively little phospholipid. Both rabbit and guinea-pig biles exhibit little evidence of hepatic cell damage, even though they are able to...

Journal of Lipid Research Volume 53, 2012 1535 Copyright © 2012 by the American Society for Biochemistry and Molecular Biology, Inc. They facilitate lipid absorption, inhibit microbe growth in the biliary tract and intestine, and function as signaling molecules that regulate energy expenditure and carbohydrate and lipid metabolism ( 2 ). The bile salt pool is maintained in an enterohepatic circ...

Studies have been made in the rat of the elimination of 210Pb in the bile after the intravenous injection of ioo ,ug. of lead. Four groups of rats were used: in two the bile was drained by cannulation of the bile duct, and in the other two the bile flow to the duodenum was interrupted by ligation or division of the bile duct. The radioactivity of the bile, of the intestinal walls, and of the in...

Bile acids are essential for the solubilization and transport of dietary lipids and are the major products of cholesterol catabolism. Results presented here show that bile acids are physiological ligands for the farnesoid X receptor (FXR), an orphan nuclear receptor. When bound to bile acids, FXR repressed transcription of the gene encoding cholesterol 7 alpha-hydroxylase, which is the rate-lim...

244 gallbladder. Like smooth mnscle, canalicular bile tlow is energy dependent. Agents tltat alter the antot, nt of ATI' in the cell sap have a similar effect on bile tlow rate. The rate of bile flow is modulated b)' a hormotte-receptor interaction attalogous to tire contraction-relaxation c)'cle of smooth muscle in which cAMI' and Ca + + are tire chief determinants. The same determinants are t...

BACKGROUND & AIMS Cholestasis promotes endoplasmic reticulum (ER) stress in the liver, however, the effect of ER stress on hepatic bile acid metabolism is unknown. We aim to determine the effect of ER stress on hepatic bile acid synthesis and transport in mice. METHODS ER stress was induced pharmacologically in C57BL/6J mice and human hepatoma (HepG2) cells. The hepatic expression of genes co...