Berberine and palmatine are two of the main bioactive components in Huangbai, a major Chinese medicinal herb. The current methods to extract these compounds usually involving the usage of inorganic acid and base, are not only complex and time-consuming, but have a low selectivity. In this paper, it was reported that hexane, ethyl acetate and dichloromethane were tested to extract berberine and ...

OBJECTIVE This study is to investigate the effect and mechanism of berberine on vascular endothelial cell injury. METHODS The isolated aortic endothelial cells were divided into negative control group, spontaneous hypertension group, and berberine group (1.25, 2.5, and 5 μmol/L berberine). CCK-8 assay was performed to detect cell proliferation. Annexin V-FITC flow cytometry and Hochest33342/P...

OBJECTIVE Defects in insulin/IGF-1 signaling stimulate muscle protein loss by suppressing protein synthesis and increasing protein degradation. Since an herbal compound, berberine, lowers blood levels of glucose and lipids, we proposed that it would improve insulin/IGF-1 signaling, blocking muscle protein losses. RESEARCH DESIGN AND METHODS We evaluated whether berberine ameliorates muscle at...

BACKGROUND Oligodendrocytes, the myelinating glial cells of central nervous system, are highly vulnerable to ischemic-induced excitotoxic insult, a phenomenon in which calcium overload triggers cell death. Berberine is an alkaloid extracted from medicinal herbs as Coptidis Rhizoma with several pharmacological effects like inhibition of neuronal apoptosis in cerebral ischemia. METHODS We exami...

background: leishmaniasis has been identified as a major public health problem in tropical and sub-tropical countries. the present study was aimed to investigate antileishmanial effects of various extracts of berberis vulgaris also its active com-poenent, berberine against leishmania tropica and l. infantum species on in vitro ex-periments. methods : in this study in vitro antileishmanial activ...

AIMS Endothelial dysfunction is a key event that links obesity, diabetes, hypertension, and cardiovascular diseases. The aim of the present study was to examine the protective effect of the alkaloid drug berberine against hyperglycemia-induced cellular injury and endothelial dysfunction. METHODS AND RESULTS In both cultured endothelial cells and blood vessels isolated from rat aorta, berberin...

Berberine is one of the main alkaloids found in the Chinese herb Huang lian (Rhizoma Coptidis), which has been reported to have multiple pharmacological activities. This study aimed to analyze the molecular targets of berberine based on literature data followed by a pathway analysis using the PANTHER program. PANTHER analysis of berberine targets showed that the most classes of molecular functi...

Background. Urate crystals-induced inflammation is a critical factor during the initiation of gouty arthritis. Berberine is well known for its anti-inflammatory activity. However, the underlying effects of berberine on monosodium urate crystals-induced inflammation remain obscure. Objectives. This study is set to explore the protective effect and mechanism of berberine on monosodium urate cryst...

INTRODUCTION Temporal lobe epilepsy (TLE) is a long lasting neurological disorder in which patients suffer from spontaneous seizures. New treatments with novel mechanisms of action are needed to help those patients whose seizures are resistant to available drugs. In this study, we investigated the possible neuroprotective effect of berberine in an intrahippocampal kainate model of TLE in rat. ...

BACKGROUND Berberine is known to improve glucose and lipid metabolism disorders, but it poorly absorbed into the blood stream from the gut. Therefore, the exact underlying mechanism for berberine is still unknown. In this study, we investigated the effect of berberine on glucose metabolism in diabetic rats and tested the hypothesis that berberine acts directly in the terminal ileums. METHODS ...