The influence of sixteen base triplet changes at a single position within a pur.pur.pyr triple helix was examined by affinity cleaving. For the 15 base pair target site studied here, G.GC, A.AT and T.AT triplets stabilize a triple helix to a greater extent than the other 13 natural triplets (pH = 7.4, 25 degrees C). Weaker interactions were detected for the C.AT, A.GC and T.CG triplets. The abs...

introdouction: the mapping of ghost images of the maxilla and the nasal cavity, which are complex structures, is very important. the position of objects that create a ghost image can differ when using various devices. the purpose of this investigation was to study the mapping of ghost images of the maxilla and the nasal cavity using a cranex d digital panoramic machine. materials and methods: t...

This investigation of the codon context of enterobacteria, plasmid, and phage protein genes was based on a search for correlations between the presence of one base type at codon position III and the presence of another base type at some other position in adjacent codons. Enterobacterial genes were compared with eukaryotic sequences for codon context effects. In enterobacterial genes, base usage...

The anticodon loop of tRNA contains a number of conserved or semiconserved nucleotides. In most tRNAs, a highly modified purine is found at position 37 immediately 3' to the anticodon. Here, we examined the role of the base at position 37 for tRNA(Phe) binding to the A site of Escherichia coli ribosomes. Affinities and rate constants of A-site binding of native yeast peptidyl-tRNA(Phe) with hyp...

Nuclear receptors are ligand-dependent transcription factors responsible for controlling differentiation, growth and development of higher eukaryotes. Three amino acids within the recognition alpha-helix of the DNA-binding domain of the nuclear receptors constitute the so-called "P-box" which determines response element specificity. In the ultraspiracle (Usp) protein, which together with EcR fo...

This paper analyzes the nucleotide sequences of three viruses: Kunjin, west Nile, and yellow fever. Each virus has one long open reading frame of greater than 10,200 nucleotides that codes for four structural and seven nonstructural genes. The Kunjin and west Nile viruses are the most closely related pair, when assessed on the basis of matches between their nucleotide sequences. As would be exp...

fragment length polymorphism (RFLP) method to analyze these 4 polymorphisms. For the detection of 608TTG GTG, we used primer 5 -AATACAGTGTTTTATATCTAAAG-3 (forward: nt 1 to 23) and 5 -CTGGATCCAGGGGCTGCTCG-3 (reverse: nt 219 to 190). A mismatched base (underlined) was introduced at the 3 -end base of the reverse primer to create a TaqI restriction enzyme cutting site in combination with the base ...