BACKGROUND Panicum hallii Vasey (Hall's panicgrass) is a compact, perennial C4 grass in the family Poaceae, which has potential to enable bioenergy research for switchgrass (Panicum virgatum L.). Unlike P. hallii, switchgrass has a large genome, allopolyploidy, self-incompatibility, a long life cycle, and large stature-all suboptimal traits for rapid genetics research. Herein we improved tissue...

In an attempt to ascertain the effects of mechanical stimulation on callus in the early stage of bone fracture healing, a tibial fracture was induced in rats and mechanical stimulation applied to the fractures. The callus was then measured quantitatively, while the fractures were analyzed both radiographically and histologically. Following the induction of a closed transverse fracture in the ti...

Lycopene β-cyclases are key enzymes located at the branch point of the carotenoid biosynthesis pathway. However, the transcriptional regulatory mechanisms of LCYb1 in citrus with abundant carotenoid accumulation are still unclear. To understand the molecular basis of CsLCYb1 expression, we isolated and functionally characterized the 5' upstream sequences of CsLCYb1 from citrus. The full-length ...

Leaf and Cotyledon explants of Withania somnifera (L). Dunal were used to evaluate the effect of different growth regulators on the in vitro direct shoot and root initiation methods. Four different explants were used to establish callus shoot and root direct regeneration. In the first experiment leaf segments were cultured on MS basal supplemented with 2,4 – Dichlorophenoxyacetic acid (2,4 – D,...

Radopholus similis and Pratylenchus coffeae were reared on callus and roots developed from citrus leaves. Callus formed best when leaf petioles were immersed in Astatula fine sand and the leaves were sprayed daily with 4 ppm 2,4-D solution and maintained at 25 or 30 C. The nematodes completed one generation in 20 days at 25 C. Highest populations of R. similis (1,127) occurred after 50 days, an...

Some physiological and biochemical changes were measured between embryogenic and non-embryogenic callus obtained from Cardiospermum halicacabum. Combination of auxin with cytokinin was more favourable for high amount of callus formation. 2,4-D played a key role in triggering somatic embryo formation. Embryogenic callus had more total carbohydrate and starch contents, total free amino acids, nuc...

Contemporary locking plates promote biological fixation through indirect reduction techniques and by elevating the plate from the bone. They have improved fixation strength in osteoporotic bone. Periarticular locking plates are rapidly being adopted for bridge plating of periprosthetic femur fractures. When these plates are used for indirect reduction and bridge plating osteosynthesis, fracture...

Crataegus aronia is a wild medicinal plant belonging to the Rosaceae family and has been known for the medicinal activity of its extracts due to the high levels of flavonoids and phenolic compounds. Recently, in vitro culture of plant cells, callus and microshoots is widely used to maximize production of plant secondary metabolites. In this study, the effect of different light intensity levels ...

abstract this experiment was conducted for optimizing a suitable medium for callus induction, regeneration and rooting of anthurium. leaf and petiol explants of antadra and casino varieties were used for callus production. ms medium was supplied with growth regulators (naa, ba, kin and iba). a complete randomized design with 3 replication was used in this expriment. result indicates that variet...

effects of culture medium containing hormonal compositions on phenotypic and chromosomal variations of sugarbeet cultured tissues were examined. experiments were carried out in different nacl levels. cytological studies were conducted on calli originated from suspension culture. hypocotyl of seven day old seedlings of the two diploid lines were cultured in ms medium containing hormonal composit...