The Epstein-Barr virus (EBV) is an attractive candidate pathogen for rheumatoid arthritis (RA) because it is persistent, ubiquitous, and has profound effects on the immune system (for review, see Tosato and Blaese'). Its persistence is due to its ability to remain latent in two cell types-the B lymphocyte and the salivary gland epitheliumlboth of which could be envisaged as possible sources for...

The Epstein-Barr virus (EBV) is an attractive candidate pathogen for rheumatoid arthritis (RA) because it is persistent, ubiquitous, and has profound effects on the immune system (for review, see Tosato and Blaese'). Its persistence is due to its ability to remain latent in two cell types-the B lymphocyte and the salivary gland epitheliumlboth of which could be envisaged as possible sources for...

We have previously shown by far-Western blotting that the Epstein-Barr virus nuclear antigen 2 (EBNA-2) both binds to a cellular protein of 130 kDa and histone H1, with the complex between EBNA-2 and p130 being tighter than between EBNA-2 and histone H1. Here we demonstrate that the N terminus of EBNA-2, which was previously shown to be necessary for transformation of B lymphocytes by EBNA-2, i...

Epstein-Barr virus (EBV), a human herpes virus with oncogenic potential, persists in B lymphoid tissues and is controlled by virus-specific cytotoxic T lymphocyte (CTL) surveillance. On reactivation in vitro, these CTLs recognize EBV-transformed lymphoblastoid cell lines (LCLs) in an HLA class I antigen-restricted fashion, but the viral antigens providing target epitopes for such recognition re...

The frequencies and levels of antibodies to Epstein-Barr virus (EBV)-specific antigens were determined in paired sera and synovial fluids from patients with rheumatoid arthritis (RA) and in sera from patients with other connective tissue diseases; i.e., systemic lupus erythematosus, progressive systemic sclerosis, and osteoarthritis (OA). The specimens were also tested for the presence of antib...

The Epstein-Barr nuclear antigen 3 (EBNA3) family of proteins, comprising EBNA3A, EBNA3B, and EBNA3C, play pivotal roles in the asymptomatic persistence and life-long latency of Epstein-Barr virus (EBV) in the worldwide human population. EBNA3-mediated transcriptional reprogramming of numerous host cell genes promotes in vitro B cell transformation and EBV persistence in vivo. Despite structura...

Two distinct cultures derived from a lymphoid cell line designated NAB were characterized immunologically, morphologically, and cytogenetically. Both cultures were positive for Epstein-Barr nuclear antigen. NAB I cultures were negative for virus capsid antigen and early antigen and were not affected by treatment with 5-iododeoxyurdine. NAB II cultures were positive for virus capsid antigen and ...