A novel amperometric immunosensor for determination of human serum chorionic gonadotrophin (HCG) was constructed by immobilization of HCG with titania sol-gel on a glassy carbon electrode and the direct electrochemistry of horseradish peroxidase (HRP) labeled to HCG antibody (HRP-anti-HCG). The morphologies of the HCG membrane were characterized to be chemically clean, porous and homogeneous. H...

Angiogenesis and vascular remodeling are crucial processes in tumor invasion and metastasis as well as in embryo implantation and normal development of the placenta. We have previously shown that hCG expressed in trophoblast and various malignant tumors promotes cellular motility and that uterine endothelium expresses hCG/LH receptor in vivo. In this study hCG was proposed to promote angiogenes...

El objetivo del trabajo fue determinar el contenido de energía metabolizable y digestibilidad íleal aparente aminoácidos en harina calmar gigante (Dosidicus gigas) (HCG) como fuente proteína dieta para pollo engorda. Experimento 1: 96 pollos (1 a 21 días edad, iniciación, crecimiento finalización), alimentados con dietas testigo, 8 16% (HCG), analizando composición química, perfil HCG, variable...

Purpose: Trophoblastic cancers (Choriocarcinoma, testicular and ovarian germ cell malignancies) secrete hyperglycosylated hCG and non-trophoblastic cancers secrete hyperglycosylated hCG free βsubunit.Both are structural variants of hCG independent to the hormone hCG. They are autocrines acting on a transforming growth factor β-II (TGF-β-II), receptor not acting on the hormone receptor. Here the...

We have obtained high spectral resolution (R = 45900) Fabry-Perot velocity maps of the Hickson Compact Group HCG 31 in order to revisit the important problem of the merger nature of the central object A+C and to derive the internal kinematics of the candidate tidal dwarf galaxies in this group. Our main findings are: (1) double kinematic components are present throughout the main body of A+C, w...

Using pseudopregnant rat ovaries, the possibility was examined whether hCG-induced early desensitization of the LH/hCG receptor was accompanied by changes in the physical state of membranes. Thirty min after a single s.c. injection of 75 IU hCG, the hCG-responsive adenylylcyclase activity was reduced, whereas hCG binding to ovarian membranes was still normal. Membrane lipid rigidity, as determi...

Human chorionic gonadotropin (hCG), a heterodimeric hormone consisting of an alpha (a) and a beta (β) subunit, is used as a marker for the diagnosis of pregnancy, congenital defects, and choriocarcinoma. After excluding the common causes of elevated serum hCG, laboratory identification of false-positive or true results assists in guiding clinical management. Options include testing urine for hC...

In an attempt to localize topographic domains specific to native human chorionic gonadotropin (hCG), we studied the discontinuous antigenic regions recognized by a monoclonal anti-hCG antibody designated as C8 which binds only to hCG and does not cross-react with either the free hCG-alpha and hCG-beta subunits or other glycoprotein hormones. Using two-site monoclonal immunoradiometric assays (M...

Objective: To compare the efficacy of progesterone alone and progesterone plus human chorionic gonadotrophin in the management of threatened abortion. Study design: Quasi experimental Duration & settings of study: The study was conducted in the department of Obstetrics & Gynaecology Unit-I, at Bahawal Victoria Hospital, Bahawalpur from 01-11-2007 to 31-10-2008. Material & method: Total 60 patie...

A clonal strain of human lung tumor cells in culture (ChaGo), derived from a bronchogenic carcinoma, synthesizes and secretes large amounts of alpha (alpha) and a comparatively lower level of beta (beta) subunit of the glycoprotein hormone, human chorionic gonadotropin (HCG). ChaGo cells lost their characteristic anchorage-independent growth phenotype in the presence of anti-alpha-HCG antibody....