Sequence analysis of the 330-kb chlorella virus PBCV-1 genome revealed an open-reading frame, A94L, that encodes a protein with significant amino acid identity to Glycoside Hydrolase Family 16 b-1,3-glucanases. The a94l gene was cloned and the protein was expressed as a GST-A94L fusion protein in Escherichia coli. The recombinant A94L protein hydrolyzed the b-1,3-glucose polymer laminarin and h...

Abstract The effectiveness of the yeast Rhodotorula glutinis was evaluated in field experiment for control gray rot disease on strawberry caused by fungus Botrytis cinerea. results showed that all treatments R. led to a significant superiority inducing systemic resistance and an increase vegetative, productivity quality indicators three cultivars, compared Prado fungicide treatment pathogenic o...

induced resistance to pathogen is one of the ways which plants use against biotic stresses. pathogenesis-related proteins(pr), including chitinase and β-1,3-glucanase synthesized by the plant in response to pathogenic infection. in this investigation, the expression of two isoforms: acidic and basic of chitinase and β-1,3-glucanasegenes were analyzed at 0, 6, 12, 24, 48 and 72 hours after funga...

understanding the function of trichoderma species in the control of phytophthora drechsleri in pistachio orchards is very important.  in this study, the effects of liquid extra-cellular secretions and volatile compounds secreted by 27 isolates of trichoderma harzianum, t. crassum, t. koningii, t.aureoviride, t. asperellum, t. brevicompactum, t.longibrachiatum and t. virens were investigated on ...

We studied the effect of constitutive expression of pathogenesis-related proteins (PRs) in tobacco plants on vesicular-arbuscular mycorrhiza. Tobacco lines genetically transformed to express various PRs constitutively under the control of the cauliflower mosaic virus 35S promoter of tobacco were examined. Immunoblot analysis and activity measurements demonstrated high levels of expression of th...

BACKGROUND Killer yeasts have been used to combat contaminating wild yeasts in food, to control pathogenic fungi in plants, and in the medical field, to develop novel antimycotics for the treatment of human and animal fungal infections. Among these killer yeasts, Tetrapisispora phaffii (formerly known as Kluyveromyces phaffii) secretes a glycoprotein known as Kpkt that is lethal to spoilage yea...

1. A cellobiohydrolase component was isolated from a Penicillium funiculosum cellulase preparation by chromatography on DEAE-Sephadex, and purified by isoelectric focusing. 2. Purified in this way, the enzyme was homogeneous as judged by electrophoresis on sodium dodecyl sulphate/polyacrylamide gels and isoelectric focusing in polyacrylamide gels. 3. Acting in isolation, the enzyme had little h...

Avocado is an important tropical fruit with high commercial value, but has a relatively short storage life. In this study, the effects of cold shock treatment (CST) on shelf life of naturally ripened and ethylene-ripened avocado fruits were investigated. Fruits were immersed in ice water for 30 min, then subjected to natural or ethylene-induced ripening. Fruit color; firmness; respiration rate;...