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Biswas, C., Chen, S., Halliday, C., Kennedy, K., Playford, E., Marriott, D., Slavin, M., Sorrell, T., Sintchenko, V. (2017). Identification of genetic markers of resistance to echinocandins, azoles and 5-fluorocytosine in Candida glabrata by nextgeneration sequencing: a feasibility study. Clinical Microbiology and Infection, 23(9), 676.e7-676.e10. <a href="http://dx.doi.org/10.1016/j.cmi.2017.0...

The Vitek 2 yeast susceptibility test was evaluated by testing 122 Candida isolates against fluconazole and voriconazole. Excellent categorical agreement with the CLSI broth microdilution method was observed (97.5% for both the azoles). Moreover, the Vitek 2 system was able to identify all but 2 of 59 investigated fluconazole-resistant organisms.

The first examples of transannular ring transformations in azirino-fused eight-membered O,N- or S,N-heterocycles involving selective aziridine ring opening and medium-sized ring contraction are described, which provide an access to functionalized 1,4-benzox(thi)azines or 1,3-benzox(thi)azoles.

Oral yeast carriage was studied in 312 Mexican subjects. Candida albicans was the most frequent species, but other Candida spp. were isolated from 16.5 to 38.5% of patients. Colonization did not correlate with CD4+ number or viral load, but highly active antiretroviral therapy reduced the frequency of candidiasis. Most isolates were susceptible to fluconazole, but 10.8% were resistant to one or...

Synthesizing azoles from ortho-substituted anilines, CO2 and H2 has proved difficult due to the low nucleophilicity of which hinders their N-formylation, i.e., first step reaction. Herein, we demonstrate that R3SnX Lewis acids (LA), N-methylmorpholine (NMM) or DBU polyethyleneimine (PEI) N-formylmorpholine efficiently catalyse synthesis benzimidazole other by reductive coupling nucleophilic ami...

Fungi transcriptionally upregulate expression of azole efflux pumps and ergosterol biosynthesis pathway genes when exposed to antifungal agents that target ergosterol biosynthesis. To date, these transcriptional responses have been shown to be dependent on the presence of the azoles and/or depletion of ergosterol. Using an inducible promoter to regulate Neurospora crassa erg11, which encodes th...

OBJECTIVES To investigate the role of transmembrane segment 11 (TMS11) of Candida albicans drug resistance protein (Cdr1p) in drug extrusion. METHODS We replaced each of the 21 putative residues of TMS11 with alanine by site-directed mutagenesis. The Saccharomyces cerevisiae AD1-8u(-) strain was used to overexpress the green fluorescent protein tagged wild-type and mutant variants of TMS11 of...

Community-onset (CO) candidemia, defined as a positive blood culture taken at or within 2 days of hospital admission, represents a distinct clinical entity associated with substantial morbidity and mortality. Reference MIC results from the SENTRY Antimicrobial Surveillance Program (2008-2009) were analyzed to compare the antifungal resistance patterns and species distributions from patients wit...

The in vitro activity of Schering 56592, a new azole drug, was compared with those of fluconazole and itraconazole against 103 isolates of Candida comprising 10 different species. Schering 56592 was more active than itraconazole and fluconazole, and it was active against many fluconazole-resistant isolates.