a3-year-old goat weighing 40 kg was presented to the largeanimal unit, university veterinary hospital, universiti putramalaysia with complains of in appetence, lateral recumbency,and weakness. physical examination findings were pyrexia,tachycardia, tachypnea, and pale mucous membrane. harsh andcrackles lung sound was noticed upon auscultation. the case wastentatively diagnosed as pneumonic past...

Pasteurella spp. were isolated from feral goats and free-ranging bighorn sheep (Ovis canadensis canadensis) in the Hells Canyon National Recreation Area bordering Idaho, Oregon, and Washington (USA). Biovariant 1 Pasteurella haemolytica organisms were isolated from one goat and one of two bighorn sheep found in close association. Both isolates produced leukotoxin and had identical electrophoret...

A selective medium (CGT medium), containing clindamycin, gentamicin, potassium tellurite and amphotericin B in 5% horse-blood agar, allowed unimpaired growth of almost all strains of Pasteurella multocida, and P pneumotropica, while inhibiting other bacteria that might be encountered in upper respiratory tract secretions. With its use, P multocida was readily detected in oral swabs from four of...

Urinary tract infections are frequently caused by Proteus mirabilis strains. In the previous studies there were defined the complete structures of O-polysaccharide parts of lipopolysaccharides from strains: P. mirabilis O3 (S1959), P. mirabilis O9 and P. mirabilis O18. In the present study it was investigated bactericidal effect of normal human serum (NHS) to P. mirabilis strains. We also focus...

Two-component systems (TCSs) play key roles in the adaptation of bacteria to environmental changes. In prototype TCSs a single phosphoryl transfer between the sensor kinase and response regulator occurs, whereas phosphorelay TCSs are characterised by a His1-Asp1-His2-Asp2 phosphorylation cascade. The TodS/TodT TCS controls the expression of a toluene degradation pathway and the TodS sensor kina...

Haemorrhagic septicaemia (HS) is a highly fatal disease in cattle and buffaloes, caused by specific serotypes of Pasteurella mul tocida. It is a primary pasteurellosis reproducible in susceptible animals with the specific organism alone. The accepted classification of Pasteurella multocida is based on the identification of its somatic and capsu­ lar antigens. On this basis two serotypes designa...

Pasteurella multocida serotype B:2 is the etiological agent of haemorrhagic septicaemia (HS) in cattle and buffaloes, a disease that causes significant economic losses to the dairy industry in India. Several killed vaccines are in uses that provide protection to limited period of time. The aim of this study was to generate an attenuated strain of Pasteurella multocida P52 by targeted gene knock...

To identify key regulators of subminimum inhibitory concentration (sub-MIC) antibiotic response in the Pasteurella multocida proteome, we applied systems approaches. Using 2D-LC-ESI-MS(2), we achieved 53% proteome coverage. To study the differential protein expression in response to sub-MIC antibiotics in the context of protein interaction networks, we inferred P. multocida Pm70 protein interac...