نتایج جستجو برای: avian leukosis
تعداد نتایج: 41374 فیلتر نتایج به سال:
DESCRIPTION YF-VAX®, Yellow Fever Vaccine, for subcutaneous use, is prepared by culturing the 17D-204 strain of yellow fever virus in living avian leukosis virus-free (ALV-free) chicken embryos. The vaccine contains sorbitol and gelatin as a stabilizer, is lyophilized, and is hermetically sealed under nitrogen. No preservative is added. The vaccine must be reconstituted immediately before use w...
A transplantable cell line was isolated from a liver tumor induced by the MC29 strain of avian leukosis virus. It was found that its ability to develop into a tumor when inoculated in recipient birds (both intramuscularly and in the wing web) was dependent on a morphological alteration which took place after the cells had been in culture for some time. The transplantable cell exhibited unusual ...
Salmonella typhimurium was invariably isolated from our J strain murine leukosis. Immunization of D103 mice with either inactivated Salmonella typhimurium or the cell-free extract of leukosis inhibited the transplantation of leukosis. The adoptive immunization of D103 mice with spleen cells of Strong A mice immunized with either Salmonella or the cell-free extract of leukosis inhibited the tran...
The caseinolytic activity of several strains of Rous sarcoma virus (RSV), conditional and nonconditional mutants of RSV, and nontransforming avian leukosis viruses was investigated. Only those viruses capable of transforming chick fibroblasts in vitro induced lysis of casein incorporated into an agar overlay. Lysis produced distinct clear areas in the turbid casein-agar gel which allowed a quan...
The Sloan-Kettering viruses (SKVs) are a group of transforming retroviruses that were isolated from chicken embryo cells which had been infected with the avian leukosis virus transformation-defective Bratislava 77 (tdB77). Each of the SKV isolates was shown to contain multiple genomes of different sizes indicating the presence of several viruses in addition to tdB77. To identify and characteriz...
Avian leukosis virus-derived vectors were constructed with an internal transcriptional promoter and various 3' non-coding sequences. Deletions were introduced into the downstream U3 long terminal repeat (LTR) to obtain self-inactivation of LTR-mediated transcription after one round of replication. However, 3' non-coding sequences appeared to determine not only self-inactivation of the vectors b...
Avian leukosis virus (ALV)-induced osteopetrosis is associated with the accumulation of unintegrated viral DNA in osteoblasts. Viruses constructed from the DNAs of an osteopetrosis-inducing ALV (Br2l) and a non-osteopetrosis-inducing ALV (RAV-0) have been used to test for the role of viral genes in the induction of osteopetrosis. Our results map osteopetrotic potential to a 1,400-base-pair regi...
An oligonucleotide complementary to the splice donor sequence of the 1.8 kb gene family produced from the BamHI-H region of Marek's disease virus (MDV) DNA inhibited the proliferation of the MDV-derived lymphoblastoid cell line, MDCC-MSB1 (MSB-1), but not that of the avian lymphoid leukosis-derived lymphoblastoid cell line, LSCC-BK3. Colony formation in soft agar was also inhibited by treatment...
In the absence of infectious subgroup E avian leukosis-sarcoma (ALS) viruses, some chicken embryo cells contain in their cell membrane the envelope glycoprotein of subgroup E viruses (gpE) (Hanafusa et al. 1973). The partial expression of subgroup E virus was first recognized as chick-cell-associated helper factor (chfl, which provided the helper function for the envelope-defective Bryan high-t...
Infection of avian bone marrow with strain MC29 leukosis virus in vitro resulted in infection, elaboration of virus, and growth of characteristic cells. Newly liberated virus with the infectious and neutralization properties of strain MC29 agent and growth of the cells were demonstrable 48 hr and 5-6 days respectively after infection. Studies by light and electron microscopy revealed similariti...
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