Background: Tapentadol is a novel centrally acting analgesic. There is no method for the determination of drug content in dosage form without any interference of any excipients and without using the diethylamine content in the mobile phase with UV detection. Methods: A simple, precise, and accurate new reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and valid...

Brewer’s spent grain is the residue left after separation of wort during beer-brewing process. Although this by-product has been reported to have a high protein content, it normally treated as waste. This work aims isolate bioactive hydrolysates from BSG, and then explore their bioactivities. Two groups (A B) BSG were subjected extraction using alkaline method at pH 12, where group A was pre-tr...

BACKGROUND The FilmArray Respiratory Panel (RP) detects multiple pathogens, including Bordetella pertussis. The multiplex PCR system is appropriate for a core laboratory or point of care due to ease of use. The purpose of this study is to compare the analytical sensitivity of the FilmArray RP, which targets the promoter region of the B. pertussis toxin gene, with the Focus real-time PCR assay, ...

A new, rapid, economical and isocratic reverse phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of eptifibatide acetate, a small synthetic antiplatelet peptide, in bulk drug substance and pharmaceutical dosage forms. The developed method was validated as per of ICH guidelines. The chromatographic separation was achieved isocratically on C18 colum...

Some [(5Z)-(5-arylalkylidene-4-oxo-2-thioxo-1,3-thiazolidin-3-yl)]acetic acids were prepared as potential antifungal compounds. The general synthetic approach to all synthesized compounds is presented. Lipophilicity of all the discussed rhodanine-3-acetic acid derivatives was analyzed using a reversed phase high performance liquid chromatography (RP-HPLC) method. The procedure was performed und...

The main objective of this research work was to evaluate the variation in nutritional profile, antioxidant, anti-proliferative and antibacterial activities selected species Brassica. Five locally grown Brassica (cauliflower, broccoli, red cabbage, white cabbage Chinese cabbage) were collected from Ayub Agriculture Research Institute (AARI), Faisalabad, Pakistan. Polyphenolic rich extracts these...