Beef heart muscle has been found to contain an enzyme which will rapidly and directly reduce metmyoglobin in vitro. Reduction rates are far greater than any previously reported for nonspecific or nonenzymatic systems. The enzyme is NADH-dependent and requires the presence of ferrocyanide ion for in vitro assay. The artificial electron carriers, dichlorophenolindophenol and methylene blue, are n...

Epidermal cholesterol biosynthesis is regulated by barrier function. We quantitated the amount and activation state (phosphorylation-dephosphorylation) of the rate-limiting enzyme, 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, in epidermis before and after barrier disruption. In murine epidermis we found high enzyme activity (1.75 +/- 0.02 nmol/min per mg protein). After acute barr...

Thc natural flavin of lactate oxidase, FMN, was removed and replaced by the synthetic flavin 2-thioriboflavin 5’phosphate (Zthio FMN). Despite the differences in properties of the flavins, including an oxidation-reduction potential some 80 mV more positive than that of normal flavin the 2-thio-FMN enzyme behaves in practically all respects like the native enzyme. It catalyzes the oxidative deca...

Besides the catalytic ability, many enzymes contain conserved domains to perform some other physiological functions. However, sometimes these conserved domains were unnecessary or even detrimental to the catalytic process for industrial application of the enzymes. In this study, based on homology modeling and molecular dynamics simulations, we found that Bacillus subtilis aminopeptidase contain...

pretreatment of adults of iraq strain of afhstephenst with a sub-letlial dose of pcrmethrin resulted in a 65% enliancemenl of tolerance to this insecticide. similarly, pre-exposure of dub-lpr larvae to a sub-lethal dose of pcrmethrin resulted in increased tolerance to pcrmethrin. for the sensitive ind-s strain, the inductive effect was smaller. tiie results of bioassays of resistant and suscept...

Monoamine oxidase (MAO, E.C. 1.4.3.4) activity was determined by spectrophotofluorimetric assay of the reaction product of the artificial substrate kynuramine in platelets from venous blood samples from 50 outpatients with glaucomas and 47 sex- and age-matched controls. No significant differences were found between MAO activity in control subjects and that in subjects with glaucomas grouped acc...

S ince the identification of the first artificial deoxyribozyme (DNA enzyme, DNAzyme) in 1994, significant progress has been made in our fundamental understanding of DNA’s capabilities as a catalyst. Many efforts have also used deoxyribozymes as critical components within analytical applications. This review addresses some of the recent developments in the area of DNA catalysis, with focus on d...

Versipelostatin (VST) is an unusual 17-membered macrocyclic polyketide product that contains a spirotetronate skeleton. In this study, the entire VST biosynthetic gene cluster (vst) spanning 108 kb from Streptomyces versipellis 4083-SVS6 was identified by heterologous expression using a bacterial artificial chromosome vector. Here, we demonstrate that an enzyme, VstJ, catalyzes the stereoselect...

Genetic programming (GP) offers a generic method of automatically fusing together classifiers using their receiver operating characteristics (ROC) to yield superior ensembles. We combine decision trees (C4.5) and artificial neural networks (ANN) on a difficult pharmaceutical data mining (KDD) drug discovery application. Specifically predicting inhibition of a P450 enzyme. Training data came fro...

Most of the supports for enzymeimmobilization are artificial materials, and chemical reactions are also required to immobilize the enzymes in most cases.1] These support materials and chemicals for immobilization must not be allowed to contaminate final products, especially foods or drugs. The requirements to achieve this are tedious, costly, and may have the wrong effects on enzyme activities....