Apolipoprotein (apo) B-100, the protein constituent of low density lipoproteins (LDL), is the determinant responsible for LDL binding to the apoB,E(LDL) receptor on cells. The current study was designed to identify the region(s) of apoB-100 that interact with the apoB,E(LDL) receptor. Apolipoprotein B-100 was fragmented by thrombin digestion, and the isolated fragments (T2, T3, T4) were recombi...

OBJECTIVE LDL oxidation is believed to play an important role in the development of atherosclerosis, and oxidized LDL particles have been shown to become targets for the immune system. Immunization of animals with oxidized LDL results in reduction of atherosclerosis, suggesting an atheroprotective effect of this immune response. METHODS AND RESULTS Using a polypeptide library covering the com...

To study the mechanisms of discrimination between various forms of vitamin E, four normal subjects, one patient with lipoprotein lipase deficiency, and three patients with abnormal apolipoprotein B-100 production were given an oral dose containing three tocopherols labeled with differing amounts of deuterium (2R,4'R,8'R-alpha-(5,7-(C2H3)2)tocopheryl acetate (d6-RRR-alpha-tocopheryl acetate), 2S...

In the present study, the relative positions of 11 anti-apolipoprotein B monoclonal antibodies have been mapped onto the surface of human low density lipoproteins by electron microscopy. As the epitopes recognized by these antibodies have been previously located on the primary sequence of apoB, these data provide a map of the configuration of the protein on the surface of the LDL. The first 89%...

Aim: To determine the association between serum and aqueous apolipoprotein (Apo) A1 Apo B levels B/A1 ratio in diabetic retinopathy.
 Material Method: This cross-sectional prospective study included 63 patients with or without retinopathy 38 control subjects who underwent cataract surgery. The groups were as follows; healthy (Group 1), 2), non-proliferative 3), proliferative 4). Serum dete...

Apolipoprotein B (apo B) is the integral apolipoprotein of chylomicrons, very-low-density lipoproteins (VLDL), intermediate-density and low-density lipoproteins. It is heterogeneous, occurring in two forms. In humans, the larger apo B-100 is formed by the liver in VLDL and the smaller apo B-48 is produced by the intestines in chylomicrons. In the rat, apo B-48 is also found in liver VLDL. Lee e...

Studies of truncated apoB peptides in human subjects with familial hypobetalipoproteinemia, as well as of puromycin-generated spectra of nascent apoB peptides in rat and hamster liver, suggest that a minimum size is required for N-terminal fragments of apoB to be efficiently assembled into full-sized VLDL. We report here results of experiments undertaken to examine this phenomenon in greater de...