Bacterial endotoxin is a potent inducer of the serum amyloid protein (apo-SAA), a high density lipoprotein (HDL) apoprotein. In a study of the induction of apo-SAA and the structure of apo-SAA-rich lipoprotein particles in mice, we have observed that, following intraperitoneal administration of Salmonella typhosa lipopolysaccharide (50 micrograms), plasma apo-SAA levels rose from base-line leve...

Liver-directed gene transfer offers new perspectives for the treatment of inherited metabolic diseases and disorders of lipoprotein metabolism. Potent expression cassettes for transgenes in the liver may optimize gene transfer efficiency and improve the therapeutic index of gene transfer vectors. An E(1)-deleted adenovirus comprising the hepatocyte specific 256-base pair (bp) human apolipoprote...

The binding of specific plasma lipoproteins to the high affinity cell surface receptors of fibroblasts is determined by the presence of specific apoproteins, the apo-B of low density lipoproteins and the apo-E of certain high density lipoproteins. Despite the observations which indicated that the recognition site responsible for receptor interaction resides exclusively with the B or E apoprotei...

Lp[a] is a lipoprotein whose plasma concentration is highly correlated with cardiovascular disease. Its protein moiety, apoLp[a], consists of two large polypeptides, apo[a] and apo B. The possible contribution of Lp[a] to atherosclerosis in saphenous vein aortocoronary bypass grafts was studied in a population of patients undergoing coronary re-bypass surgery. The vein graft tissue levels of ap...

To explore mechanisms underlying triglyceride (TG) accumulation in livers of chow-fed apo E-deficient mice (Kuipers, F., J.M. van Ree, M.H. Hofker, H. Wolters, G. In't Veld, R.J. Vonk, H.M.G. Princen, and L.M. Havekes. 1996. Hepatology. 24:241-247), we investigated the effects of apo E deficiency on secretion of VLDL-associated TG (a) in vivo in mice, (b) in isolated perfused mouse livers, and ...

Clinical studies Indicate that levels of apolipoprotein A-l (apo A-l), the major protein moiety of the high density lipoprotein (HDL) particle, may provide more Information concerning the risk of future cardiovascular disease than do levels of HDL cholesterol (HDL-C). Therefore, the relationship of HDL-C to apo A-l levels was examined In a blraclal sample of 2849 5to 17-year-olds. The mean HDL-...

A simple procedure for phenotyping apolipoprotein (apo) E directly from plasma has been developed for use in the lipid clinic laboratory. In this new method, 10 microL of serum or plasma is pretreated with neuraminidase (EC 3.2.1.18), which removes the sialic acid residues from apo E and eliminates additional bands, thereby ensuring correct phenotype assignment. After a rapid delipidation step,...