Colistin resistance in Acinetobacter spp. is increasing, resulting potentially untreatable nosocomial infections. Plasmid-mediated colistin of particular concern due to its low fitness cost and potential transferability other bacterial strains species. This study investigated the mechanism a clinical nosocomialis isolate from Cape Town, South Africa. A colistin-resistant A. was identified blood...

34 35 RepB is the pMV158-encoded protein that initiates rolling-circle 36 replication of this promiscuous plasmid. Availability of RepB is rate-limiting for 37 the plasmid replication process, and therefore the repB gene encoding the 38 protein is subjected to strict control. Two trans-acting plasmid elements, CopG 39 and the antisense RNAII, are involved in controlling the synthesis of the ini...

The purpose of this study was to characterize a collection of 103 multidrug resistance IncF plasmids recovered from Escherichia coli of food producing and companion animals between 2003 and 2012. A total of 103 incF plasmids were characterized using an established PCR-based IncF replicon sequence typing (RST) system to identify FII, FIA, and FIB (FAB) groups. Plasmids were also analyzed using-r...

The not-for profit foundation n-Lorem seeks to provide experimental antisense oligonucleotide treatments patients with ultra-rare disease free for their entire lifetime.

the biodegradation of secondary amines is particularly important due to their propensity for conversion either chemically or microbiologically to n-nitrosamines which are potent carcinogens. in this research, a weak gram-positive organism was isolated from river and identified as mycobacterium. this mycobacterium grows slowly and effectively utilizes piperazine as the sole source of organic, ca...

We determined sites in X ell mRNA that are cleaved by RNase III in the presence of X OOP antisense RNA, using a series of OOP RNAs with different internal deletions. In OOP RNA-cll mRNA structures containing a potential region of continuous double-stranded RNA bounded by a non-complementary unpaired region, RNase III cleaved the ell mRNA at one or more preferred sites located 10 to 14 bases fro...

Plasmid DNA chromatography is a powerful field in constant development and evolution. The use of this technique considered mandatory the production an efficient safe formulation to be applied for plasmid-mediated gene therapy. Concerning this, search ideal chromatographic support/ligand combination motivated scientist pursue continuous improvement on plasmid performance, looking progression lig...

gene therapy is in its development stage as a novel method for cancer treatment. liposomes look promising as gene delivery vectors; however, investigations have shown that these vesicles are not doing well in some cases. it was decided here to investigate the possibility of augmentation of liposomal gene delivery by chemical penetration enhancers.cationic liposome containing antisense oligonucl...

background and objectives: bacteria, most prevalently the pseudomonas species possess high capacity to utilize and de- grade petroleum hydrocarbons and are classified as the hydrocarbonoclastic microorganisms. many species of the genus pseudomonas are notorious for their aerobic degradation capacity, extracellular enzyme production and are metabolically versatile organisms capable of utilizing ...