conclusions the results of this study illustrated the high prevalence of antibiotic resistant biofilm-producing s. epidermidis strains in this hospital, which could be a reservoir for antibiotic resistance genes. results the results of the biofilm assay revealed that 82% of strains produced black colonies on congo red agar plates and 68% were able to attach strongly to polystyrene microplates. ...

Background and aims: Salmonella spp. infect cold-blooded and warm-blooded animals and maycause a worldwide zoonotic disease, salmonellosis, in infected animals. Rodents can be Salmonellacarriers without any signs of salmonellosis. The frequency of salmonella contamination, the presence ofvirulence genes (SpvR and SpvB) and antibiotic resistance pattern of isolated Salmonella w...

Bacteria with intrinsic resistance to antibiotics are a worrisome health problem. It is widely believed that intrinsic antibiotic resistance of bacterial pathogens is mainly the consequence of cellular impermeability and activity of efflux pumps. However, the analysis of transposon-tagged Pseudomonas aeruginosa mutants presented in this article shows that this phenotype emerges from the action ...

Objectives Effluents contain a diverse abundance of antibiotic resistance genes that augment the resistome of receiving aquatic environments. However, uncertainty remains regarding their temporal persistence, transcription and response to anthropogenic factors, such as antibiotic usage. We present a spatiotemporal study within a river catchment (River Cam, UK) that aims to determine the contrib...

conclusions this study represented an increasing rate of mdr p. aeruginosa in burn and wound samples. efflux mexab genes were detected in all mdr and pdr strains. the p. aeruginosa strain isolated from burn cases showed higher drug resistance and pdr resistance was only noted in a burn sample. materials and methods one-hundred and fifty p. aeruginosa were isolated from burn and wound infections...

UNLABELLED Antibiotic resistance is a worldwide health risk, but the influence of animal agriculture on the genetic context and enrichment of individual antibiotic resistance alleles remains unclear. Using quantitative PCR followed by amplicon sequencing, we quantified and sequenced 44 genes related to antibiotic resistance, mobile genetic elements, and bacterial phylogeny in microbiomes from U...

Droplet digital PCR revealed antibiotic resistance genes, metal and intI1 across all pipe biofilm sample types (biomass surfaces, corrosion tubercles, under tubercles).

Antibiotic resistance genes are typically isolated by cloning from cultured bacteria or by polymerase chain reaction (PCR) amplification from environmental samples. These methods do not access the potential reservoir of undiscovered antibiotic resistance genes harboured by soil bacteria because most soil bacteria are not cultured readily, and PCR detection of antibiotic resistance genes depends...