background: toxoplasmosis is one of the prevalent parasitic diseases in the world with 10-40 percent of people having it as a latent infection. in cases of renal transplants, the condition either presents itself as a result of reactivation of the latent infection resulting in the weakness of immune system in a recipient or happens when a recipient simply contracts the condition from the donor t...

this study aimed to evaluate freeze-dried sera as an alternative to non-freeze dried for detection of anti-leishmania infantum antibodies over the course of 11 months using the direct agglutination test (dat).altogether, 60 serum samples (30 from humans and 30 from dogs) were collected from various geographical locations in iran. all the collected sera were pooled and each pooled serum sample c...

An in-house enzyme-linked immunosorbent assay (ELISA) was developed in this study to detect circulating IgG antibodies to peptide antigens derived from baculoviral IAP repeat-containing protein 5 isoform 2 (BIRC5) and myc proto-oncogene protein (MYC) in non-small cell lung cancer (NSCLC). Student's t-test revealed that circulating anti-MYC IgG levels were significantly increased in patients wit...

Background: Glaucoma is a progressive optic neuropathy and is one of the leading causes of blindness worldwide. Different factors have been contributed in the pathogenesis of glaucoma including H. pylori infection. Objective: To determine the levels of anti-H. pylori IgG antibody in the aqueous humor of patients with pseudoexfoliation and primary open angle glaucoma, in comparison with age and ...

Non-enzymatic glycosylation of proteins is one of the key mechanisms in the pathogenesis of diabetic complications. Glycation of IgG is of special interest due to its possible influence on the functionality of immunoglobulins and overall immuno-competence. The aim of this study was to clarify more details of in vitro glycation of IgG and to study the effect of this modification on its interatio...

OBJECTIVES To determine the prevalence and clinical correlation of autoantibody to peroxiredoxin (Prx) I, an antioxidant enzyme, in patients with systemic sclerosis (SSc). METHODS Serum samples from SSc patients (n = 70) and healthy controls (n = 23) were examined by ELISA using human recombinant Prx I. The presence of anti-Prx I antibody was further evaluated by immunoblotting analysis. To d...

The relationship between Chlamydophila pneumoniae (Cp) infection and peripheral arterial occlusive disease (PAD) was studied by analyzing clinical samples from 95 patients with PAD and 100 controls. The following investigations were conducted: IgG and IgA against lipopolysaccharide (LPS) and against purified Cp-specific antigens from elementary bodies (EB) with ELISA; anti-EB IgG, with MIF; Cp ...

Dilated cardiomyopathies (DCM) are a major cause of mortality in patients with systemic lupus erythematosus (SLE). Immune responses induced by human parvovirus B19 (B19) are considered an important pathogenic mechanism in myocarditis or DCM. However, little is known about Th17-related cytokines in SLE patients with DCM about the linkage with B19 infection. IgM and IgG against B19 viral protein,...

Previous experiments showed that the physiologic IgG anti-F(ab')2 antibody suppresses the response of human autoreactive B cells. In the present study, we analyzed the IgG anti-F(ab')2 antibody in 293 patients with cold agglutination (CA). Their average IgG anti-F(ab')2 titer was not much different (211 +/- 8.3) from that of 279 healthy persons (195 +/- 6.7). However, CA patients with high anti...

We assessed SARS-CoV-2 anti-spike immunoglobulin G (anti-S-IgG) levels among healthcare workers (HCWs) after BNT162b2 vaccination. The anti-S-IgG titers of study participants were measured every three months, a week before, and weeks each This compared the short-term immune response to second vaccination in four HCWs who received first two doses six months apart (due acute allergic-like adverse...