Methylation by Ava methylases in Escherichia coli increases the efficiency to transfer of Tn5 in pBR322bla:: Tn5 from E. coli to Anabaena sp. strain PCC 7120 by conjugation. Following conjugation, Tn5 but not pBR322 sequences were found at many different positions in the Anabaena chromosome. This procedure was used to mutagenize, tag, and clone a previously unrecognized gene required for nitrog...

A thiobencarb dose of 3 mg L(-1) reduced the protein content of Anabaena variabilis, whereas it elevated the carbohydrate content. Measurements of Anabaena growth, photosynthetic activity, and respiration rate revealed that the high dose of phosphate (0.53 mM) exerted no additional toxic effect to thiobencarb toxicity. Recovery of Anabaena cells from the inhibitory effect of thiobencarb occurre...

The strengths of several promoters were assessed in the cyanobacterium Anabaena PCC 7120 by fusing them to luxAB, encoding bacterial luciferase. Two promoters, Ptac and PpsbA, with sequences nearly identical to consensus Escherichia coli sigma 70 promoters, gave as high or higher expression than the strong Anabaena promoter, Prbc. Pnpt, the natural promoter driving expression of the kanamycin-r...

In the present study, Synechococcus elongatus and Anabaena, two cyanobacterial species were cultured using different media conditions such as ASN III, modified BG-11, BBM for enrichment of biomass lipid productivity. The experimental result clearly shows that BG 11 was efficient cost-effective medium both isolated Anabaena. influence organic carbon sources on productivity selected studied when ...

The addition of DL-7-azatryptophan (AZAT), a tryptophan analog, to continuous cultures of Anabaena sp. strain CA grown with 10 mM nitrate as the nitrogen source resulted in the differentiation of heterocysts. Analysis of the intracellular amino acid pools of Anabaena sp. strain CA after the addition of AZAT showed a marked decline in the intracellular glutamate pool and a slight increase in the...

Four types of VLSI architectures for the hardware realization of the FLOS-CM algorithm are introduced in this paper. Each architecture is appropriate for a particular environment. The FLOS-CM algorithm is found to be amenable for implementation using logarithmic arithmetic. A logarithmic architecture is shown to require up to 50% less area and be 14% faster than a linear fixed-point arithmetic ...

Background Virus infection is the main triggering event for the development of acute rhinosinusitis and human influenza A virus ranks among the most frequent viral causes of infection. Influenza neuraminidase, a key enzyme in viral replication, spread, and pathogenesis, is the primary target in prevention and treatment of influenza infection. Sinupret, a herbal medicinal product composed of Gen...

The kdpFABC operon of Escherichia coli, coding for the high-affinity K(+) transport system KdpFABC, is transcriptionally regulated by the products of the adjacently located kdpDE genes. The KdpD protein is a membrane-bound sensor kinase consisting of a large N-terminal domain and a C-terminal transmitter domain interconnected by four transmembrane segments (the transmembrane segments together w...

To choose a suitable DNA marker to authenticate the botanical origins of Flos Lonicerae Japonicae and Flos Lonicerae, seven candidate DNA bar codes (i.e., RBCL, MATK, PSBA-TRNH, ITS2, ITS, TRNL intron, and TRNL-F intergenic spacer) were tested on forty-four samples of LONICERA JAPONICA and its closely related species using the DNA barcoding method. We found that all seven candidate bar codes yi...