Amylase is one of the earliest characterized enzymes and has many applications in clinical and industrial settings. In biotechnological industries, the amylase activity is enhanced through modifying amylase structure and through cloning and expressing targeted amylases in different species. It is important to understand how engineered amylases can survive from generation to generation. This stu...

α-Amylases are widespread enzymes that catalyze endohydrolysis of long α-1,4-glucan chains such as starch and glycogen. The highest amylolytic activity was found in 5th instar nymphs and midgut of the predatory bug, Andrallus spinidens F. (Hemiptera: Pentatomidae). The α-amylase was purified following a three-step procedure. The purified α-amylase had a specific activity of 13.46 U/mg protein, ...

In six conscious dogs with gastric and duodenal cannulas, secretin (164 pmol. kg(-1). h(-1) iv) was given to provide a flow of pancreatic juice of approximately 1 drop/s. Amylase activity was measured in each drop before and after rapid intravenous injection of caerulein (7.4 pmol/kg) or intraduodenal injection of L-tryptophan (1 mmol), sodium oleate (3 mmol), and HCl (3 mmol). All experiments ...

The alpha-amylase (alpha-1,4-glucan 4-glucanohydrolase, EC 3.2.1.1) of Bacillus subtilis strain W23 is less negatively-charged than the alpha-amylase of B. amyloliquefaciens strain F, as determined by electrophoretic mobility in polyacrylamide gel at pH 8.6. The alpha-amylase of strain W23 is immunologically unrelated to the alpha-amylase of strain F, as judged by lack of cross-reaction in Ouch...

Of alpha-amylases and other starch-digesting enzymes from bacteria and plants.ess was supplemented with bacterial and fungal amylases or scalded flour. Immunochemical characteristics of alpha-amylase sensitization.Alpha-amylase production and its activity was compared in two different. Bacterial amylases are resistant to high temperatures, they can active in wide pH.fungal amylascs lowest, liqu...

The ontogenesis and specific activities of pancreatic (trypsin, chymotrypsin, amylase and lipase) and intestinal enzymes (alkaline phosphatase, aminopeptidase N) were investigated in Kutum (Rutilus frisii Kutum) from the onset of exogenous feeding (3 day after hatching, DAH) to the juvenile stage at 50 DAH. Trypsin- and chymotrypsin-specific activity showed similar patterns and increased with l...

We report a case of primary bronchogenic adenocarcinoma, complicated by pleural effusion, in which very high pleural amylase activity was found, whilst serum amylase was normal. Isoamylase determination showed a salivary-type amylase. Concerning the origin of this enzyme, ultrastructural study of the malignant cells obtained from the pleural fluid suggested a local amylase synthesis. The pathop...

CONTEXT Although the synthesis and secretion of pancreatic enzymes are dependent on the composition of diet, little is known about the long-term adaptation of the exocrine pancreas to the chronic intake of high-fat, low-carbohydrate diets. OBJECTIVE The effects of long-term (48-weeks) high-fat, low-carbohydrate feeding on the intracellular activity as well as on the secretory activities of th...

A bacterium, isolated from rabbit’s waste and identified as Cellulomonas sp., had cellulase and thermostable α-amylase activity when grown on wheat bran. Maximum activity of thermostable α-amylase was obtained by adding 3% soluble starch. However, soybean oil (1 ml l) could increase the production of α-amylase and cellulase in wheat bran. The α-amylase was characterized by making a demonstratio...

in this study, the effects of dietary egg lecithin on digestive enzymes and body biochemical composition of juveniles mesopotamichthys sharpeyi was evaluated. four experimental diets including control diet (with 0% egg lecithin) and three diets containing 2%, 4% and 6% egg lecithin were used. at the end of the experiment, digestive enzymes activity (lipase, amylase and alkaline phosphatase) and...