Addition of 2 mg of Pb2+/g of soil concident with or after amendment with starch or maltose resulted in 75 and 50% decreases in net synthesis of amylase and alpha-glucosidase, respectively. Invertase synthesis in sucrose-amended soil was transiently reduced after Pb2+ addition. Amylase activity was several times less sensitive to Pb2+ inhibition than was enzyme synthesis. In most cases, the rat...

Macroamylasemia results from the complexing of serum a-amylase (EC 3.2.1.1;a-1,4-glucan 4-glucanohydrolase) to a high-molecular-weight protein or proteins (macroamylase) and is characterized by hyperamylasemia and low urinary amylase clearance, associated with normal renal function. Recently a simplified detection method has been described for macroamylasemia, in which a small column of Sephade...

The secretion of salivary α-amylase is influenced by adrenergic regulation of the sympathetic nervous system and the hypothalamic-pituitary-adrenal axis; thus, exercise affects the levels of salivary α-amylase. Granger et al published a review in 2007 that focused attention on salivary α-amylase. In addition, a portable system for monitoring salivary α-amylase activity was launched in Japan at ...

BACKGROUND There have been many reports describing hyperamylasemia, with a salivary-type amylase phenotype, in patients with malignant tumors and/or multiple myelomas. In contrast, we have discovered and characterized a sialyl salivary-type amylase from multiple myeloma and/or lung cancer cells. This paper reports the first association of sialyl salivary-type amylase with ovarian cancer, discov...

Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield of α-amylase in B. subtilis although huge efforts have been made to address this secretion bottleneck. In this question-oriented review, every effort is made to answer the following que...

The physiological and molecular bases for contrasting [alpha]-amylase phenotypes were examined in germinating seeds of two barley (Hordeum vulgare L.) cultivars, Morex and Steptoe. Morex is a high-quality malting barley that develops high [alpha]-amylase activity soon after germination. Steptoe is a feed barley that develops only low [alpha]-amylase activity levels during this period. The expre...

The oral commensal bacterium Streptococcus gordonii interacts with salivary amylase via two amylase-binding proteins, AbpA and AbpB. Based on sequence analysis, the 20-kDa AbpA protein is unique to S. gordonii, whereas the 82-kDa AbpB protein appears to share sequence homology with other bacterial dipeptidases. The aim of this study was to verify the peptidase activity of AbpB and further explo...

Waste oil industry has a great potential use, as 77% oil yield are an untapped waste that still has a high economic value as a base for the oleochemical industry, one of which is β-carotene. The study was conducted to optimize the extraction of carotenoids from Crude Palm Oil (CPO) and Palm Oil Mill Effluent waste (POME) conducted by column chromatography and extraction method with a single enz...

Amylase is one of the earliest characterized enzymes and has many applications in clinical and industrial settings. In biotechnological industries, the amylase activity is enhanced through modifying amylase structure and through cloning and expressing targeted amylases in different species. It is important to understand how engineered amylases can survive from generation to generation. This stu...

the side effects associated with the usage of synthetic antidiabetic drugs make it imperative to search for alternative drugs from medicinal plants. therefore, this study was aimed at evaluating the α-amylase and α-glucosidase inhibitory potential of calotropis procera leaf, as well as its possible mode of inhibiting these enzymes. acetone, aqueous and ethanolic extracts of c. procera leaf was ...