Restriction fragment length polymorphisms (RFLP) have been identified at the goat. The objective of the present study was to determine polymorphisms of calpastatin locus in Raini and Tali goats of the goats in Kerman Province, improving meat quality traits superior meat quality by selection. Calpastatin gene effect on quality and tenderness meat, to identify different genotype of this gene was ...

A novel application of fluorescence in situ hybridization (FISH) to isolated nuclei is described. The method detects gene amplification and chromosome aneuploidy in extracted nuclei from paraffin-embedded tissue of human cancer with greater sensitivity and specificity than existing FISH methods. In this study, the method is applied to signal detection of the HER-2/neu (c-erbB-2) gene, whose amp...

the staphylococci are important pathogenic bacteria causing various infections in animals and human. hemolysin is one of the virulence factors of coagulase-positive (cps) and coagulase-negative staphylococci (cns). the aims of the study were to characterize hemolysins of staphylococcus spp. isolated from human and bovine origin, phenotypic- and genotypically. characterization of hemolysin pheno...

background and objectives: loop-mediated isothermal amplification is a novel nucleic acid amplification assay providing as a simple diagnostic tool for rapid identification of microbial diseases in developing countries. in this study, a lamp assay was established for yersinia enterocolitica , a leading cause of acute enterocolitis in young children. materials and methods: lamp assay was establi...

Background High-copy genomic sequences could be used as PCR targets for the detection of Plasmodium infections, providing increased sensitivity over single- or low-copy genes. Mitochondrial genomes malaria parasites are present in multiple copies a single mitochondrion, and each parasite has many mitochondria. Here, we describe development seven species-specific qPCR assays diagnosis vivax falc...

Bridge Technology is an amplification technique in which pairs of primers are immobilized on a solid support, allowing amplification only at the location of the primer pair spot. The technique has diagnostic potential since an array of primer pairs, each specific for a different pathogen, can be used with a diagnostic sample without inter-pair interactions that plague the development of multipl...

We describe an homogeneous luminescence immunoassay for "total" estrogens in enzymically hydrolyzed urine from nonpregnant women. The antiserum, raised against estriol-16,17- dihemisuccinate conjugated to bovine serum albumin, specifically bound the C-19 steroids carrying the estrogen-characteristic phenolic group. 17 beta-Estradiol conjugated with aminobutylethyl isoluminol was used to monitor...

INTRODUCTION Effect of bariatric surgery on health-related quality of life (HRQOL) varies greatly. This might be caused by the diversity in questionnaires used to assess HRQOL and the weight loss of the studied population. This study assesses the relationship between weight loss and HRQOL in primary Roux-en-Y gastric bypass (RYGB) patients by using an obesity-specific (impact of weight on quali...