A new S9 family aminopeptidase derived from the actinobacterial thermophile Acidothermus cellulolyticus was cloned and engineered into a transaminopeptidase by site-directed mutagenesis of catalytic Ser(491) into Cys. The engineered biocatalyst, designated aminolysin-A, can catalyze the formation of peptide bonds to give linear homo-oligopeptides, hetero-dipeptides, and cyclic dipeptides using ...

Well-defined molecular brushes bearing polypeptides as side chains were prepared by a "grafting through" synthetic strategy with two-dimensional control over the brush molecular architectures. By integrating N-carboxyanhydride ring-opening polymerizations (NCA ROPs) and ring-opening metathesis polymerizations (ROMPs), desirable segment lengths of polypeptide side chains and polynorbornene brush...

A new pathway to nano-sized hollow-sphere particles from six-arm star polymers with an amphiphilic core-corona structure, synthesized in a four-step-procedure by means of reversible addition-fragmentation chain transfer (RAFT) polymerization is presented, in order to achieve more stable and versatile nano-container systems, which could be applied in the fields of drug delivery or catalyst stora...

The optimal conditions for regio- and stereoselective epoxide ring opening of N,N-disubstituted 1,2-epoxy-3-aminocyclopentanes by different nucleophilic reagents have been developed. The substituents on the nitrogen atom in the epoxide precursor and the orientation of the oxirane ring are crucial for the reaction outcome. Thus, treatment of (1RS,2SR,3SR)-1,2-epoxy-3-(N,N-dibenzylamino)cyclopent...

Polyurethane foams currently dominate the global foam market. Nevertheless, main drawback of these remains use toxic isocyanate reagents. To overcome this issue, several ways have been explored. The most promising route appears to be aminolysis 5-membered cyclic carbonates leading formation polyhydroxyurethanes (PHUs). However, lack reactivity systems using C5-cyclic limits application range PH...

The surface of polylactic acid fiber (PLAF) was treated with self - manufactured starch nanocrystals (SNCs) to improve its interfacial adhesion the matrix. Determination optimum dispersion conditions SNCs by transmittance. effects direct oscillatory deposition, chemical grafting modification, and concentration on treatment effect PLAF bonding matrix were investigated. results showed that succes...