Nickel-chromium (Ni-Cr) alloys used in fixed prosthodontics have been associated with type IV Ni-induced hypersensitivity. We hypothesised that the full-thickness human-derived oral mucosa model employed for biocompatibility testing of base-metal dental alloys would provide insights into the mechanisms of Ni-induced toxicity. Primary oral keratinocytes and gingival fibroblasts were seeded onto ...

The objectives of this study were to develop a serum-free system for culturing canine oral keratinocytes, the construction and characterization of a canine ex vivo produced oral mucosa equivalent (EVPOME), and transduction green fluorescent protein (GFP) into keratinocytes as a post-grafting tracking marker. Dissociated canine buccal mucosa keratinocytes were cultured in a chemically defined se...

Background. While the benefits of using acellular dermal matrices (ADMs) in breast reconstruction are well described, their use has been associated with additional complications. The purpose of this study was to determine if ADM thickness affects complications in breast reconstruction. Methods. A retrospective chart review was performed including all tissue expander based breast reconstructions...

Dental implants are becoming the treatment of choice to replace missing teeth, especially if the adjacent teeth are free of restorations. When minimal bone width is present, implant placement becomes a challenge and often resulting in recession and dehiscence around the implant that leads to subsequent gingival recession. To correct such defect, the author turned to soft tissue autografting and...

OBJECTIVE Providing adequate soft tissue cover while preventing wound breakdown and infection can present a challenge when repairing large meningomyeloceles. Adding an extra barrier to protect the underlying dural elements in the event of complications should lower the morbidity and mortality associated with large repairs, which are at risk of dehiscence and subsequent exposure of the neural el...

RESULTS: The majority of flaps were for lower extremity trauma wounds (74), followed by upper extremity trauma wounds (8), scalp tumor defects (4), and one torso wound. There were 7 flap losses, but of these, 4 were due to noncompliance with postoperative immobilization/elevation orders; excluding these 4 failures for which the NPD was clearly not a factor, the flap success rate was 96.4%. Skin...

OBJECTIVE Different surgical procedures have been proposed for the treatment of gingival recessions. The goal of this study was to compare the clinical results of gingival recession treatment using Subepithelial Connective Tissue Graft and an Acellular Dermal Matrix Allograft. MATERIALS AND METHODS The present study was performed on 5 patients with 9 bilateral Miller's class I or II gingival ...

A problem maxillofacial surgeons face is a lack of sufficient autogenous oral mucosa for reconstruction of the oral cavity. Split-thickness or oral mucosa grafts require more than one surgical procedure and can result in donor site morbidity. Skin has disadvantages of adnexal structures and a different keratinization pattern than oral mucosa. In this study, we successfully assembled, ex vivo, a...