Kanamycin, penicillin, and streptomycin were injected individually into air cells of newly set chicken and turkey hatching eggs, and assay was made throughout the incubation period to determine the location, potency, and duration of efficacy. By 24 hours, each antibiotic had diffused throughout the albumen. By six days, penicillin residues could not be detected, whereas kanamycin or streptomyci...

Certain of the experiments reported in the preceding paper suggested that propagation of influenza virus in or on the cells lining the aUantoic sac of the chick embryo can be divided into two major phases. According to this suggestion, the first phase would correspond to the production of some incomplete non-infectious forms or building blocks of the virus which, in the second phase, are conver...

Human influenza viruses are more efficiently isolated by inoculating patient samples into the amniotic rather than the allantoic cavity of embryonated chicken eggs. This type of cultivation selects virus variants with mutations around the hemagglutinin (HA) receptor binding site. To understand the molecular basis of these phenomena, we investigated the abundances of sialic acid (SA) linked to g...

Hemagglutination (HA) and hemagglutination inhibition (HI) tests for avian influenza (AI) virus (H5N1) were standardized varying various factors like erythrocytes from different species, type of diluent, incubation temperature and incubation period. The virus was propagated in embryonated chicken eggs (9-11 days). The allantoic fluid (AF) was harvested 36 hours post incubation and was confirmed...

Introduction Purification of mouse hepatitis virus (MHV) has not been succeeded until recently. It has been stated that mice infected with MHV produced the interferon-like substances within the host cells, and it produced remarkable disturbance of typical infectious cycles and symptoms (ALTUCCI 1959). FANTON1 (1964) reported that he could be able to purify the MHV ,partially by the use of fluor...

Nucleic acid sequence-based amplification with electrochemiluminescent detection (NASBA/ECL) is an isothermal technique allowing rapid amplification and detection of specific regions of nucleic acid from a diverse range of sources. It is especially suitable for amplifying RNA. A NASBA/ECL technique has been developed allowing the detection of RNA from avian influenza virus subtype H7 derived fr...

Scrapie is a naturally occurring prion (PrP) disease causing a fatal neurodegenerative disorder in sheep and goats. Previous studies suggest that scrapie is transmitted naturally through exposure to the scrapie agent in wasted placentas of infected ewes. This study determined the distribution and biochemical properties of PrP cellular (PrP-C) and the distribution of PrP scrapie (PrP-Sc) in repr...

Background and Aims: Infectious bronchitis virus (IBV) causes an acute, highly contagious respiratory and kidney disease of chickens which results in significant economic losses in commercial broilers, layers and breeders. Rapid identification of IBV serotypes involved in respiratory complex is a problem in the differential diagnosis. Materials and Methods: In this study, a single and mul...