Since hypophosphatasia was first described (Rathbun, 1948), other cases which have been recognized have raised the possibility that this defect of alkaline phosphatase is an inherited inborn error of metabolism. Schneider and Corcoran (1950) first suggested that this was an inherited disorder when two cases were found in one family, and this was corroborated by Sobel, Clark, Fox and Robinow (19...

A very rapid method of agar gel electrophoresis on glass slides, together with a superior visualization technique employing simultaneous coupling of a hydrolysed naphthol substrate, have been developed for the identification of the tissues of origin of serum alkaline phosphatase. Combined with L-phenylalanine inhibition, specific for the intestinal enzyme, and heat inactivation, specific for th...

Electrophoresis of some serum samples on polyacrylamide gel, followed by staining for alkaline phosphatase (EC 3.1.3.1), produces a band of activity at the gel origin. This high-Mr band consists of liver membrane fragments containing alkaline phosphatase and other enzymes. Alkaline phosphatase is closely associated with phosphatidylinositol in liver plasma membranes, and we have found that phos...

The aim of this study was to determine the effect of mineral fertilization on the activity of alkaline and acid phosphatase and content of available phosphorus in soil under winter wheat cultivation. The fi rst factor of experiment was the fertilization with P, K, Mg, Ca and S in six levels: 1 (P, K, Mg, Ca and S), 2 (K, Mg, Ca and S), 3 (P, Mg, Ca and S), 4 (P, K, Ca and S), 5 (P, K, Mg, Ca), ...

Background: Vitamin D deficiencies are highly prevalent globally, but the correlation between serum alkaline phosphatase and vitamin D3 deficiency among Indian children is seldom investigated. To describe the correlation of vitamin D3 levels and serum alkaline phosphatase levels among children admitted with malnutrition to the pediatric wards of a tertiary care center. Methods: This descriptiv...

In the method of Rosalki and Foo (Clin Chem 1984;30:1182-6) bone and liver isoenzymes of alkaline phosphatase (EC 3.1.3.1) are quantified by using wheat-germ agglutinin (WGA). I suggest standardizing the procedure by using a WGA concentration that precipitates half of the alkaline phosphatase activity of serum pooled from an equal number of healthy women and men. By applying knowledge of the pr...

By WILLIAM KERPPOLA, M.I).* P HOSPHATASE can be demonstrated in tissues by means of reliable histochemical methods. Such a method! w’as described for alkaline phosphatase in 1939 by Gomori,#{176}’ and in the same year by Takamatsu.2 Two years later Gomori#{176} described his method for demonstrating acid phosphatase. The principle on which these methods are based is to incubate slices of tissue...

In the kidney of the adult mouse the tubular epithelium in the most proximal (Pi) segment, corresponding roughly to the convoluted portion of the proximal tubule, shows strong alkaline phosphatase activity, and the brush border is moderately reactive to the periodic acid Schiff (PAS) method. In the more distal (P2) segment of the proximal tubule there is no alkaline phosphatase activity, and th...

As illustrated in the preceding paper (Firestone, G. L., and Heath, E. C. (1981) J. Biol. Chem. 256, 13961403), the de novo synthesis of a cell surface-localized glycoprotein that exhibits alkaline phosphatase activity was induced by treatment of mouse L-cell cultures with dibutyryl cyclic AMP. When the exposure of L-cell cultures to dibutyryl cyclic AMP was carried out in the presence of inhib...

The alkaline phosphatase from KB cells was purified, characterized, and compared to placental alkaline phosphatase, which it resembles immunologically. Two nonidentical nonomeric subunits of the KB phosphatase were found. The two subunits, which have apparent molecular weights of 64,000 and 72,000, can be separated on polyacrylamide gels containing sodium dodecyl sulfate. The Mr = 64,000 KB sub...