The mode of membrane attachment of the Rieske iron-sulfur protein from cytochrome bc1 complex of Rhodospirillum rubrum has been studied using biochemical approaches. In contrast to cytochrome c1 the bacterial Rieske protein was extracted from chromatophores using chaotropic agents (NaSCN, urea, guanidine), an alkaline pH and relatively low concentration of Triton X-100. The results presented he...

was recovered in the first protein peak in its bimolecular complex form. The final chromatography step at 4°C on a DEAE-Sephacel column at a slightly alkaline pH purified the toxin (Mr, 145,000) by separating the nontoxic protein from the complex. At least 1.5 mg of pure toxin was obtained from each liter of culture, and the toxicity was 6 x 107 50% lethal doses per mg of protein. These values ...

A significant tin deposit has been identified in the northeast part of the Late Proterozoic Arabian Shield. Cassiterite-topaz-quartz greisens are distributed over 16 km2 in flat-topped cupolas of a highly evolved, zinnwaldite-bearing alkali-feldspar granite which composes part of the silsilah ring complex (lat 26"06'N, long 4240'E). The 587 m.y. old alkali-feldspar granite is overlain by a cara...

Wheat germ agglutinin (WGA) precipitates bone type serum alkaline phosphatase (sALP) isoenzyme specifically. The precipitates are composed of the macromolecules of WGA and "bone type sALP" (WGA-ALP complex). In order to use bone type sALP as a marker in polyacrylamide gel electrophoresis (PAGE), a method to separate "bone type sALP" from the "WGA-ALP complex" was established by using N-acetyl-D...

To establish the structure of ferric ions in strongly alkaline (pH > 13) environments, aqueous NaOH solutions supersaturated with respect to Fe(III) and the solid ferric-hydroxo complex salts precipitating from them have been characterized with a variety of experimental techniques. From UV measurements, in solutions of pH > 13, only one kind of Fe(III)-hydroxo complex species was found to be pr...