نتایج جستجو برای: alkali labile phosphate
تعداد نتایج: 129053 فیلتر نتایج به سال:
The forms and dynamics of soil phosphorus can be greatly affected by land use changes, which often involve changes in vegetation cover, biomass production and nutrient cycling in the ecosystem. Present research evaluates the impact of land use change on the amount of total organic and inorganic P, labile, moderately labile and nonlabile P pools in semiarid soils of central Zagros. Samples were ...
The techniques of viscoelastometry and S1 nuclease digestion were applied to the analysis of DNA damage in rat 9L cells treated with nitrogen mustard and 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU). Results of the S1 nuclease assay permitted quantitation of the amount of single-strand (or alkali-labile) break formation as well as DNA interstrand cross-link formation. In the presence of 2% deter...
The comet assay is considered to be a rapid and sensitive procedure for quantitating DNA damage in mammalian cells. In this article, to interpret its outcomes adequately, its power to detect low level genotoxicity and factors affecting its power were reviewed. Although the development of initial lesions into alkali-labile sites and/or SSBs through repairing events is an important factor to supp...
Two substances differing in immunological behavior as well as in certain chemical properties have been isolated from soluble extracts of B. proteus X-19. Both substances appear to be polysaccharides. The first substance is precipitated from X-19 crude extracts by a relatively low percentage of alcohol and electrolytes (from one to two and a half volumes of alcohol). When purified as far as poss...
BACKGROUND Metabolic acidosis is a common complication of chronic kidney disease; current guidelines recommend treatment with alkali if bicarbonate levels are lower than 22 mMol/L. In fact, recent studies have shown that an early administration of alkali reduces progression of CKD. The aim of the study is to evaluate the effect of fruit and vegetables to reduce the acid load in CKD. METHODS W...
DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) is a procedure to detect and quantify DNA breaks in situ, on a cell-by-cell basis. A comparison between sperm nuclei versus peripheral blood leukocytes using this method demonstrated that the nucleoids from mature human sperm are 12.7 times more sensitive to alkaline denaturation than those from human peripheral blood leukocyt...
V. A. KNIVETT I954 3. [32P]Potassium dihydrogen phosphate was used to distinguish between ATP formed from ADP by the action of adenylate kinase (myokinase) and by synthesis involving inorganic phosphate. The appearance of 32P in ADP, and in both of the labile phosphates of ATP, but not in AMP, indicates the presence of adenylate kinase in extracts of Strep. faecali&. 4. The fission of citrullin...
In a previous study, we showed that the inositol pyrophosphate diphosphoinositol pentakisphosphate (IP(7)) physiologically phosphorylates mammalian and yeast proteins. We now report that this phosphate transfer reflects pyrophosphorylation. Thus, proteins must be prephosphorylated by ATP to prime them for IP(7) phosphorylation. IP(7) phosphorylates synthetic phosphopeptides but not if their pho...
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