The fatty acid composition of lipid extracts from cells of Bacillus acidocaldarius grown at temperatures of 50 to 70 C and pH values of 2 to 5 was determined by gas chromatography of the methyl esters. The most abundant fatty acids are 11-cyclohexylundecanoic and 13-cyclohexyltridecanoic, followed by anteiso- and iso-heptadecanoic; unsaturated acids are absent. Highly aerated cultures produce m...

The molecular interplay between cargo recognition and regulation of the activity of the kinesin-1 microtubule motor is not well understood. Using the lysosome adaptor SKIP (also known as PLEKHM2) as model cargo, we show that the kinesin heavy chains (KHCs), in addition to the kinesin light chains (KLCs), can recognize tryptophan-acidic-binding determinants on the cargo when presented in the con...

Bacterial isolates assigned to the species Alicyclobacillus tolerans, which occupies an intermediate position between organotrophic genus and mixotrophic Sulfobacillus, were revealed as members of acidophilic chemolithotrophic community during stirred-tank bioleaching violarite–pentlandite–chalcopyrite concentrate at 40 °C. Surprisingly, this succeeded more common iron-oxidizing after a series ...

The crystal structure of squalene-hopene cyclase from Alicyclobacillus acidocaldarius was determined at 2.9 angstrom resolution. The mechanism and sequence of this cyclase are closely related to those of 2,3-oxidosqualene cyclases that catalyze the cyclization step in cholesterol biosynthesis. The structure reveals a membrane protein with membrane-binding characteristics similar to those of pro...