New practical procedures have been developed for the enzyme-catalyzed synthesis of carbohydrates and peptides. Aldolases have been shown to be effective catalysts for the synthesis of uncommon sugars, particularly azasugars. Enzymatic methods for the large-scale synthesis of oligosaccharides have been developed with the use of glycosyltransferases coupled with the regeneration of sugar nucleoti...

BACKGROUND Chronic hypoxia causes apoptosis of cardiac myocytes, however, energy production by anaerobic glycolysis protects myocardium against hypoxia injuries. Aldolase A is a well-characterised key enzyme of the glycolysis pathway. Ghrelin, a 28-amino-acid peptide, synthesizes in the stomach and has protective roles in cardiovascular systems and also affects metabolic pathways. OBJECTIVES ...

Yeast aldolase was purified partially by Warburg and Christian (I), who found that it was inhibited by metal-binding agents and could be reactivated by addition of Fe++, Co++, or Zn++ ions. Later, this enzyme was further purified, treated with a chelating agent, and crystallized as a mercury salt (2). In this form, the enzyme was still active when tested in the presence of cysteine and Zn++ ion...

Simultaneous binding of two sequential glycolytic enzymes, phosphofructokinase and aldolase, to a microtubular network was investigated. The binding of the phosphofructokinase to microtubules and its bundling activity has been previously characterized (Lehotzky, A., Telegdi, M., Liliom, K., and Ovádi, J. (1993) J. Biol. Chem. 268, 10888-10894). Aldolase binding to microtubules at near physiolog...

The differences between the aldolase A mRNA forms seem therefore to occur through their 5‘ non-coding stretches. Several possible mechanisms leading to the expression of such different messengers could then be proposed. Two promoters, one ubiquitous and the other specific to adult fast twitch muscle, could exist. Otherwise, the same premRNA transcript might be differentially spliced, or even bo...

Aldolases derived from various sources may be differentiated into two types according to whether or not they are inhibited by metal-chelating agents (2). Warburg and Christian (3) first recognized a distinctive difference in the properties of yeast and muscle aldolase. The yeast enzyme was strongly inhibited by cyanide, pyrophosphate, cysteine, and cr ,cr’-dipyrridyl, and the inhibition was rev...