Datura metal L is an important medicinal plant containing the alkaloids like scopalamine(hyoscine),hyoscyamine(atropine) scutellaerin etc .The total alkaloids present in the callus were higher than the amount of alkaloids present in leaves .For the higher production of alkaloids the medium plays avital role.The addition of L-arginine were enhanced the alkaloid production The higher production o...

Agrobacterium sp. ATCC 31749 is an industrial strain for the commercial production of curdlan, an important exopolysaccharide with food and medical applications. Here we report the genome sequence of the curdlan-producing strain ATCC 31749. Genome sequencing is the first step toward the understanding of regulation of curdlan biosynthesis.

Transferring foreign genes into mushroom mediated by Agrobacterium tumefaciens is a standard technique in genetic engineering. Recombinant human insulin has been greatly used in the treatment of type I diabetes. The production of edible mushroom derived insulin should facilitate oral delivery. In this study we used the Agrobacterium tumefaciens mediated transformation method for the transfer an...

As aerobic chemoorganotrophs, most Agrobacterium strains will grow on a wide range of complex and defined media. Methods commonly used for the culture and storage of other chemoorganotrophs will usually work for agrobacteria as well. Problems with culture or strain maintenance will occur more frequently because of careless technique than because of strain difficulties. Here we describe a few of...

We constructed the broad-host-range plasmid pUCD800 containing the sacB gene of Bacillus subtilis for use in the positive selection and isolation of insertion sequence (IS) elements in gram-negative bacteria. Cells containing pUCD800 do not grow on medium containing 5% sucrose unless the sacB gene is inactivated. By using pUCD800, we isolated a 1.4-kilobase putative IS element from Agrobacteriu...

E Keshamma, B. Madhusudhan, Department of Biochemistry, Center for Nano Science and Technology Research, Kuvenpu University, P.G. Centre, Shivagangotri Davangere 577 002, India; S. Rohini, M. Udaya Kumar*, Department of Crop Physiology, University of Agricultural Sciences, GKVK Bangalore 560 065, India; K. S. Rao, Centre for Ecological Sciences, Indian Institute of Science, Bangalore 560012, In...

In the present study high efficiency Agrobacterium tumefaciens mediated transformation system of wheat (Triticum aestivum L.) cv. Inqilab-91 was developed by exploiting bacterial culture density and acetosyringone concentration. Agrobacterium strain EHA101 harboring binary vector pIG121Hm, containing gene for GUS activity and hygromycin resistance was used in transformation experiments. Differe...