Enteropathogenic transmissible gastroenteritis virus (TGEV), a porcine coronavirus, is able to agglutinate erythrocytes because of sialic acid binding activity. Competitive inhibitors that may mask the sialic acid binding activity can be inactivated by sialidase treatment of virions. Here, we show that TGEV virions with efficient hemagglutinating activity were also obtained when cells were trea...

Group O D-negative blood cells are universal donors in transfusion medicine and methods for converting other blood groups into this universal donor group have been researched. However, conversion of D-positive cells into D-negative is yet to be achieved, although conversion of group A or B cells into O cells has been reported. The Rh D blood group is determined by the RHD gene, which encodes a ...

1. Intact pneumococci, possessing specific antigenic powers unimpaired by cultural or other procedures, give rise to agglutinins for organisms of the homologous type and to precipitins for the type-specific carbohydrate derived from them. 2. Solutions of pneumococci free of all formed elements, but containing the carbohydrate and protein of the original cell, fail to stimulate the formation of ...

The outer membrane protein OmpU of Vibrio cholerae O1 strain 86B3 was characterized with reference to colonization of the intestine by the organism. The purified OmpU exhibited a pI of 3.6. Upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, it migrated to 38, 32, and 110 kDa when the sample was heated at 100 degrees C for 2 min, 50 degrees C for 15 min, and room temperature for 30 ...

Data are presented which enhance the idea that the influenza virus is an unstable agent. They indicate that the O-D type of variation is not a discontinuous mutation but rather is a reversible phenomenon. The O and the D forms of virus both appear to be inherent in the virus particle; the dominance of one or the other form seems to be subject to chance occurrences, but is influenced by the cond...

Analysis of a shear supernatant from flocculent, "fimbriated" Saccharomyces cerevisiae brewer's yeast cells revealed the presence of a protein involved in flocculation of the yeast cells and therefore designated a flocculin. The molecular mass of the flocculin was estimated to be over 300 kDa, as judged from sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Gel permeation chromatograph...

An encapsulated strain of Escherichia coli has been isolated which is hemolytic, pathogenic for mice, and which has served to illustrate further evidence of heterogenetic specificity. The relationship appears to be limited to the serological reactions between the colon organism and Type I antipneumococcic horse serum. Type I antipneumococcic rabbit serum failed to agglutinate the organism and n...

Although latex agglutination assays have been used for some years to diagnose thrombotic disorders, only recently has it been possible to measure specifically the products of fibrin breakdown in the presence of fibrinogen degradation products, by using monoclonal antibodies. We have evaluated a preparation of latex particles coupled to the monoclonal antibody DD-3B6/22, which is specific for cr...

Since it was demonstrated that some sera of rheumatoid arthritic patients would agglutinate human Rh-positive O-erythrocytes coated with a selected incomplete anti-Rh serum (Grubb, 1956; Waller and Vaughan, 1956), this phenomenon has been extensively investigated (for example, in Finland by Julkunen, Laine, Koskinen, and Tiilikainen, 1958). Grubb and Laurell (1956) showed that this agglutinatio...

Capsulated mutants of pneumococcus producing a capsule of soluble polysaccharide related immunologically to the C or cell wall polysaccharide of pneumococcus have been isolated from several noncapsulated variants of this organism. The capsular material of these strains reacts with antisera both to homologous strains and to noncapsulated strains of pneumococcus and with human C-reactive protein....