In this work, we demonstrate the applicability of coherent anti-Stokes Raman scattering (CARS) micro-spectroscopy for quantitative chemical imaging of saturated and unsaturated lipids in human stem-cell derived adipocytes. We compare dual-frequency/differential CARS (D-CARS), which enables rapid imaging and simple data analysis, with broadband hyperspectral CARS microscopy analyzed using an uns...

Coherent anti-Stokes Raman scattering (CARS) microscopy with high sensitivity, and high three dimensional resolution, has been developed for vibrational imaging of chemical species [1]. Due to the coherent nature of the CARS emission, it has been reported that the Epi-CARS (E-CARS) and Forward-CARS (F-CARS) signals detection depends on the size and shape of the sample [2,3]. In this paper, we i...

INTRODUCTION Some car rental companies in California and other states in the USA have established non-smoking policies for their vehicles. This study examined the effectiveness of these policies in maintaining smoke-free rental cars. METHODS A stratified random sample of 250 cars (non-smoker, smoker and unknown designation) was examined in San Diego County, California, USA. Dust, surfaces and...

Molecular contrast in optical coherence tomography (OCT) is demonstrated by use of coherent anti-Stokes Raman scattering (CARS) for molecular sensitivity. Femtosecond laser pulses are focused into a sample by use of a low-numerical-aperture lens to generate CARS photons, and the backreflected CARS signal is interferometrically measured. With the chemical selectivity provided by CARS and the adv...

We discuss the requirements for laser systems used in Coherent Anti-Stokes Raman Scattering (CARS) microscopy and particularly in its wide-field modification. While such laser parameters as wavelength, spectral width and frequency difference between pump and Stokes beams are similar for all CARS systems, requirements for pulse energy, repetition rate, pulse length and mode structure might be si...

The purpose of this study was to evaluate scores generated from the Autism Treatment Evaluation Checklist (ATEC), a parent-rated measure, and those derived from professionally completed Childhood Autism Rating Scale (CARS) evaluations. A cohort of 56 participants diagnosed with an autism spectrum disorder was used for the study, and each child was evaluated independently by the parent using the...